Relative ITS2 abundance of Saccharomyces in stool over time as a Saccharomyces cerevisiae-free diet was consumed. Relative ITS2 abundance of Saccharomyces in stool over time as a Saccharomyces cerevisiae-free diet was consumed. A logarithmically scaled chart showing the unrarefied relative abundances of Saccharomyces reads among ITS2 sequences amplified from stool DNA of a human volunteer is shown. Plant sequences were removed before analysis. The volunteer consumed a regular diet for 7 days and a Saccharomyces cerevisiae-free diet for 7 days and then resumed a regular diet. Day 8 data represent averages of results from two stool samples, and there was no sample on day 12. Thomas A. Auchtung et al. mSphere 2018; doi:10.1128/mSphere.00092-18