Pretreatment with 3,5,3′ triiodo-l-thyronine (t3): Effects on myocyte contractile function after hypothermic cardioplegic arrest and rewarming Jennifer.

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Presentation transcript:

Pretreatment with 3,5,3′ triiodo-l-thyronine (t3): Effects on myocyte contractile function after hypothermic cardioplegic arrest and rewarming Jennifer D. Walker, MD*, Fred A. Crawford, MD, Francis G. Spinale, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 110, Issue 2, Pages 315-327 (August 1995) DOI: 10.1016/S0022-5223(95)70227-X Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 1 Treatment protocol used in present study. Myocytes were initially placed in standard culture medium alone or with 80 pmol/L T3 . After 2-hour incubation period in cell culture medium with or without supplementation of T3 , one aliquot of cells from each group was subjected to 2 hours of hypothermic cardioplegic arrest, and a second aliquot from each group served as a control. In the first aliquot from each group, medium was carefully replaced with 2.5 ml of crystalloid cardioplegia solution ([K+]: 24 mmol/L; pH: 7.4; oxygen tension: >400 mm Hg). These cells were maintained at 4° C for 2 hours. In the other aliquot from each group, medium was carefully replaced with fresh cell culture medium not containing T3 and placed at 37° C for the 2-hour incubation time. At the end of this 2-hour period (normothermia or hypothermic cardioplegic arrest), myocytes were resuspended in cell medium at 37° C. Contractile function was examined at the time points designated with an X. The Journal of Thoracic and Cardiovascular Surgery 1995 110, 315-327DOI: (10.1016/S0022-5223(95)70227-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 2 Isolated myocyte profile surface area was determined by digitizing cardiocyte profiles with subsequent computer analysis of results. To determine profile surface area, minimum of 300 to 500 myocytes was measured under each of following conditions: top panel, 2 hours of normothermia; middle panel, 2 hours of hypothermic cardioplegic arrest (HCA); bottom panel, after resuspension and rewarming (R). Left panels represent myocytes measured in absence of T3. Right panels represent myocytes measured after T3 preincubation. These large sample sizes allowed for Gaussian distribution (black bell-shaped curve), thus providing means for parametric analysis. Two hours of hyperkalemic, hypothermic cardioplegic arrest caused significant reduction in myocyte volume in both panels. Subsequent reperfusion and rewarming resulted in significant increase in myocyte volume from hypothermic cardioplegic arrest values and from normothermic values. Preincubation with T3 had no effect on alterations in cell size with either hypothermic cardioplegic arrest or with subsequent rewarming. Results are summarized in Table V. The Journal of Thoracic and Cardiovascular Surgery 1995 110, 315-327DOI: (10.1016/S0022-5223(95)70227-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 2 Isolated myocyte profile surface area was determined by digitizing cardiocyte profiles with subsequent computer analysis of results. To determine profile surface area, minimum of 300 to 500 myocytes was measured under each of following conditions: top panel, 2 hours of normothermia; middle panel, 2 hours of hypothermic cardioplegic arrest (HCA); bottom panel, after resuspension and rewarming (R). Left panels represent myocytes measured in absence of T3. Right panels represent myocytes measured after T3 preincubation. These large sample sizes allowed for Gaussian distribution (black bell-shaped curve), thus providing means for parametric analysis. Two hours of hyperkalemic, hypothermic cardioplegic arrest caused significant reduction in myocyte volume in both panels. Subsequent reperfusion and rewarming resulted in significant increase in myocyte volume from hypothermic cardioplegic arrest values and from normothermic values. Preincubation with T3 had no effect on alterations in cell size with either hypothermic cardioplegic arrest or with subsequent rewarming. Results are summarized in Table V. The Journal of Thoracic and Cardiovascular Surgery 1995 110, 315-327DOI: (10.1016/S0022-5223(95)70227-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 2 Isolated myocyte profile surface area was determined by digitizing cardiocyte profiles with subsequent computer analysis of results. To determine profile surface area, minimum of 300 to 500 myocytes was measured under each of following conditions: top panel, 2 hours of normothermia; middle panel, 2 hours of hypothermic cardioplegic arrest (HCA); bottom panel, after resuspension and rewarming (R). Left panels represent myocytes measured in absence of T3. Right panels represent myocytes measured after T3 preincubation. These large sample sizes allowed for Gaussian distribution (black bell-shaped curve), thus providing means for parametric analysis. Two hours of hyperkalemic, hypothermic cardioplegic arrest caused significant reduction in myocyte volume in both panels. Subsequent reperfusion and rewarming resulted in significant increase in myocyte volume from hypothermic cardioplegic arrest values and from normothermic values. Preincubation with T3 had no effect on alterations in cell size with either hypothermic cardioplegic arrest or with subsequent rewarming. Results are summarized in Table V. The Journal of Thoracic and Cardiovascular Surgery 1995 110, 315-327DOI: (10.1016/S0022-5223(95)70227-X) Copyright © 1995 Mosby, Inc. Terms and Conditions