Statistical Treatment of Fluorescence in Situ Hybridization Validation Data to Generate Normal Reference Ranges Using Excel Functions Allison L. Ciolino,

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Statistical Treatment of Fluorescence in Situ Hybridization Validation Data to Generate Normal Reference Ranges Using Excel Functions Allison L. Ciolino, Mary E. Tang, Ron Bryant The Journal of Molecular Diagnostics Volume 11, Issue 4, Pages 330-333 (July 2009) DOI: 10.2353/jmoldx.2009.080101 Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Binomial distribution functions with 95% confidence limits indicated. A: p = 0.013, n = 100, k0.95 = 3; B: p = 0.013, n = 500, k0.95 = 11. The Journal of Molecular Diagnostics 2009 11, 330-333DOI: (10.2353/jmoldx.2009.080101) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Normal cut-off values at the 95% confidence limit calculated from the simulated validation data in Table 2 calculated by three methods. Binomial: ‘o’; Gaussian (DeMoivre-Laplace): ‘*’; and β inverse: ‘x’. The Journal of Molecular Diagnostics 2009 11, 330-333DOI: (10.2353/jmoldx.2009.080101) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions