Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study Wangde Dai,

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Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study Wangde Dai, MD, Sharon L. Hale, BS, Robert A. Kloner, MD, PhD Journal of Vascular Surgery Volume 45, Issue 4, Pages 817-820 (April 2007) DOI: 10.1016/j.jvs.2006.12.013 Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

Fig 1 Representative hematoxylin and eosin stained sections of the abdominal inferior vena cava. A, Inferior vena cava that received neonatal heart cells. Viable grafts (black arrows) are located around the inferior vena cava (red arrow). B, Inferior vena cava in a control rat that received medium only. The wall of the inferior vena cava (red arrow) is surrounded by loose connective tissue (A and B, original magnification ×40). C, Higher magnification (×400) of the boxed area in A. The transplanted cardiomyocytes (black arrows) are visible in the outer wall of the vena cava. D, Higher magnification (×400) of the boxed area in B shows the loose connective tissues around the vena cava (black arrow). Journal of Vascular Surgery 2007 45, 817-820DOI: (10.1016/j.jvs.2006.12.013) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

Fig 2 Immunohistochemical staining of the same slices as in Fig 1, A for sarcomeric actin. The grafted cardiomyocytes are organized in a circular pattern parallel to the vena cava wall, and show stronger positive brown staining and typical cross striations of sarcomere formation (red arrows) (×400). Journal of Vascular Surgery 2007 45, 817-820DOI: (10.1016/j.jvs.2006.12.013) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions