Fig. 2. Efficient HDR in primary T cells leading to introduction of a GFP expression cassette within the CCR5 locus. Efficient HDR in primary T cells leading.

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Fig. 2. Efficient HDR in primary T cells leading to introduction of a GFP expression cassette within the CCR5 locus. Efficient HDR in primary T cells leading to introduction of a GFP expression cassette within the CCR5 locus. (A) (Top) Diagram of AAV constructs used as donor templates. (Bottom) Timeline of gene-editing procedure beginning with bead stimulation of CD4+ T cells. pA, SV40 polyadenylation signal; HA, homology arm. (B) Representative flow cytometry plots showing BFP versus GFP expression at days 2 and 16 after gene editing. The percentage of live single lymphocytes within the relevant quadrant is indicated. (C) Cell viability and BFP and GFP expression over time after gene editing (n = 5). Significance was calculated using the unpaired t test with the Holm-Sidak correction for multiple comparisons; significant P values (<0.05) are in red. (D) Diagram of human CCR5 locus before and after HDR with the AAV.CCR5.GFP donor template. Primers binding outside homology arms were used to amplify gDNA from treated cells visualized on agarose gel; expected sizes of bands for unedited and edited alleles are indicated; right chart shows indel % (determined by TIDE sequencing) within the lower–molecular weight (MW) bands (n = 3). P value was calculated using the unpaired two-tailed t test. (E) Nested polymerase chain reaction (PCR) of single cells (31 to 100 cells per condition) edited using megaTAL and AAV.CCR5.GFP donor at indicted MOIs. Graph shows percentages of amplicons with a single higher–molecular weight band (biallelic HDR) and a single lower–molecular weight band (no HDR detected) or both bands (monoallelic HDR). Blythe D. Sather et al., Sci Transl Med 2015;7:307ra156 Published by AAAS