Dynamic compression of single cells

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General Information Osteoarthritis and Cartilage

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Dynamic compression of single cells A.C. Shieh, B.S.E., K.A. Athanasiou, Ph.D., P.E. Osteoarthritis and Cartilage Volume 15, Issue 3, Pages 328-334 (March 2007) DOI: 10.1016/j.joca.2006.08.013 Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 (A) Histogram of the untransformed abundance values for Gapdh. Note that the majority of cells are clustered to the left. (B) Histogram of the log-transformed abundance values of Gapdh, with an accompanying curve fit for a normal distribution. Since the transformed values are normally distributed, Gapdh expression is actually lognormally distributed. Osteoarthritis and Cartilage 2007 15, 328-334DOI: (10.1016/j.joca.2006.08.013) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Graph of the means of log-transformed relative abundances of Col2a1 for mechanically stimulated single chondrocytes. Bars that do not share at least one letter in common are statistically significantly different (P<0.05). Static compression of 100 and 50nN significantly decreased gene expression compared to controls, while dynamic compression at those levels approached or returned to control levels. Compressive forces of 25nN did not change relative to controls. Sample sizes: n=74 (control); n=36 (static, 100nN); n=9–15 (all other treatment groups). Osteoarthritis and Cartilage 2007 15, 328-334DOI: (10.1016/j.joca.2006.08.013) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Graph of the means of log-transformed relative abundances of Agc for mechanically stimulated single chondrocytes. Bars that do not share at least one letter in common are statistically significantly different (P<0.05). Static loads of 100nN and dynamic loading at 50nN significantly reduced Agc gene expression. Note that the bars for the 100nN intermittent and 50nN dynamic groups do not have error bars; all cells in these groups showed no detectable Agc gene expression, and were given an arbitrary value of −3, which was one order of magnitude beyond the detection limit of the assay. Sample sizes: n=87 (control); n=36 (static, 100nN); n=11–15 (all other groups). Osteoarthritis and Cartilage 2007 15, 328-334DOI: (10.1016/j.joca.2006.08.013) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions