Cas9/gRNA-mediated gene deficiency in DF-1 cells.

Slides:

Advertisements

Similar presentations

Searching for Genes Important in Seed Development At1g19000 At1g74840 BY: Mike Douglas.

Advertisements

Arabidopsis Thaliana A Study of Genes and Embryo Development By Garen Polatoglu.

Nephrin mutants enhance ATF6‐luciferase reporter activity

Enhanced DNA damage in IDH1 mutant glioma cells.

Homocysteine inhibits endothelial cell growth via DNA hypomethylation of the cyclin Agene by Md S. Jamaluddin, Irene Chen, Fan Yang, Xiaohua Jiang, Michael.

Characterization of the Human Platelet/Endothelial Cell Adhesion Molecule-1 Promoter: Identification of a GATA-2 Binding Element Required for Optimal Transcriptional.

Implications of somatic mutations in the AML1 gene in radiation-associated and therapy-related myelodysplastic syndrome/acute myeloid leukemia by Hironori.

by Hong Hao, Huiling Qi, and Manohar Ratnam

Measurement of fetal γ-globin expression due to KLF1 being a negative or positive regulator By: Ashria Arora.

Improvement of the intracellular interaction between anti‐RAS intrabodies and RAS antigen by the mutation of framework sequences. Improvement of the intracellular.

The N‐terminus and SH2 domain of SOCS‐1 contribute to inhibition of JAK1 and JAK2 kinase activity. The N‐terminus and SH2 domain of SOCS‐1 contribute to.

A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures Gang Wang, Na Zhao, Ben Berkhout, Atze.

REV-ERBα agonists reduce protein O-GlcNAcylation.

by Wu-Guo Deng, Ying Zhu, and Kenneth K. Wu

by Kwang-Hyun Baek, Michelle A

Measurement of fetal γ-globin expression due to KLF1 being a negative or positive regulator By: Ashria Arora.

The interferon regulatory factor ICSBP/IRF-8 in combination with PU

by Sansana Sawasdikosol, Kristin M. Russo, and Steven J. Burakoff

A new mutation in the HNF4 binding region of the factor VII promoter in a patient with severe factor VII deficiency by Josephine A. Carew, Eleanor S. Pollak,

Testing the iGM insertion module.

Schematic representation of the lbk gene, positions of gRNAs, and knock-in cassette. Schematic representation of the lbk gene, positions of gRNAs, and.

miR-335 targeted RANKL and reduced osteoclast induction in SBC-5.

Figure 2 Luciferase assays of transiently transfected HEK 293 cells with reporter constructs containing the 766-bp wild-type KCNJ18 or c.-542 T/A mutant.

Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.

Fig. 5. GUCD1 was a direct target of miR-370 in HCC

Figure 3 Mutation carrier–derived lymphoblastoid cell lines (LCLs) show decreased aconitase 2 activity and mitochondrial respiration deficiency compared.

RAD51 is essential for L. donovani.

Gang Wang, Na Zhao, Ben Berkhout, Atze T Das Molecular Therapy

Localization of selected clones in mammalian COS-7 cells.

NILV‐S/MAR‐mediated transgene expression

XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells.

Mutagenesis through Cas9/sgRNA-induced HDR

USP2a-dependent miRs deregulation modulates MYC expression.

CD137/NFATc1 Axis Was Directly Targeted by miR-145 (A) Relative mRNA expression levels of NFATc1 and CD137 in the miR-145 mimic- or inhibitor-treated group.

Structure of the MCS in backbone vectors for the BiFC plasmids.

Expression of luciferase reporter gene in various promoter sequences.

NILV‐S/MAR‐mediated transgene expression

Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-

Establishment of monoclonal SMN2-GFP reporter line in HEK293.

Volume 15, Issue 2, Pages (August 2014)

Disruption of the svkA gene encoding severin kinase.

(A) yellow cDNA comparison among wild-type and ch mutants

Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

14-3-3γ phosphorylated at S59 by Lats2 in response to UV damage.

The NF-κB-binding site of the uPAR promoter is required forβ 3-endonexin-mediated downregulation of the uPAR gene transcription. The NF-κB-binding site.

Multiple cis-acting elements in the IGRP promoter are required for maximal basal reporter gene expression in βTC-3 cells. βTC-3 cells were transiently.

miR-145 regulates IGF1R expression.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

A and B, mRNA expression for ZNF423 (A) and BRCA1 (B) for lymphoblastoid cell lines with WT/WT (8 cell lines), WT/V (7 cell lines), and V/V (8 cell lines)

Structural insights based on chimeric Alp4-GCP2 analysis.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

Interaction between the G6PC2 rs G and rs G alleles on G6PC2 promoter activity. βTC-3 cells were transiently cotransfected, as described.

Western blotting for GluR1.

Volume 113, Issue 4, Pages (May 2003)

VP16-E2 is a more potent transcriptional activator than Gal4-VP16.

Activity-dependent suppression of the proapoptotic gene Puma protects neurons. Activity-dependent suppression of the proapoptotic gene Puma protects neurons.

NF-κB superrepressor was overexpressed by means of the bicistronic retrovirus. NF-κB superrepressor was overexpressed by means of the bicistronic retrovirus.

Notch1 signaling mediates the neuronal induction of BLBP promoter transcription in glia. Notch1 signaling mediates the neuronal induction of BLBP promoter.

RhoH is underexpressed in HCL mediated by a transcriptional mechanism.

by Valentino M. Gantz, and Ethan Bier

Targeting DCLK1 by miRNA-137.

Pirh2 represses p73-dependent transactivation.

Isolation of stable XPAKD and XPCKD HeLa clones.

The effects of HDAC2 knockdown on cell-cycle proteins.

DEAR1 blocks TGF-β–induced anoikis resistance, and TGF-β and SMAD3 signal transduction. DEAR1 blocks TGF-β–induced anoikis resistance, and TGF-β and SMAD3.

HOXA11-AS acts as a ceRNA for miR-1297.

Investigation of reactivity of D14 HLA-A

Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a.

Presentation transcript:

Cas9/gRNA-mediated gene deficiency in DF-1 cells. Cas9/gRNA-mediated gene deficiency in DF-1 cells. (A) Structure of the C2EIP gene and selection of the gRNA site. (B) Flowchart for the SSA activity assay. (C) Results of the SSA activity assay show high luciferase activity after transfection with the vector containing gRNA-3. (D) Right: results of the T7EI assay show a clear band at approximately 250 bp and gene knockout. Left: The effect of transfection in DF-1. (E) Alignment of TA clone sequences. (F) TA clone sequencing of monoclonal cells shows homozygous mutations in #3, #4, #7, and #9, and heterozygous mutations in the other cell lines. (G) Results of Western blot analysis show very low expression of C2EIP protein in monoclonal cells after transfection with the Cas9/gRNA plasmid. Qisheng Zuo et al. G3 2016;6:1787-1792 ©2016 by Genetics Society of America