Endolysosomal proteins are exported from the Golgi complex in two distinct populations of transport carriers. Endolysosomal proteins are exported from.

Slides:

Advertisements

Similar presentations

Movie 1 Top and side view volume renderings of filopodia in a HeLa cell expressing mEmerald-Lifeact.The high speed of the dithered mode of lattice light-sheet.

Advertisements

An analysis of Plk4 centriolar dynamics.

Fanny Jaulin, Xiaoxiao Xue, Enrique Rodriguez-Boulan, Geri Kreitzer

Calcium regulates ERK nuclear association, but not its activation.

Volume 54, Issue 4, Pages (May 2014)

Mitofilin cluster distribution is denser in the perinuclear mitochondria. Mitofilin cluster distribution is denser in the perinuclear mitochondria. (A)

MOSPD2 is a putative tail‐anchored protein

Volume 9, Issue 5, Pages (May 2016)

Volume 4, Issue 5, Pages (September 2011)

CCL21 localizes to T cell zone FRCs in young and aged spleens.

STED microscopy of MINOS

Dan Zhang, Aleksandar Vjestica, Snezhana Oliferenko Current Biology

EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling Sarah Gierke, Torsten Wittmann Current Biology

Volume 15, Issue 8, Pages (May 2016)

Marker identification and quantification by StrataQuest, and confocal analysis of EV-containing tissue sections. Marker identification and quantification.

Fig. 4 TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV release in murine MSCs. TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV.

Volume 23, Issue 8, Pages (May 2018)

Model depicting the sorting of endolysosomal proteins in the Golgi complex. Model depicting the sorting of endolysosomal proteins in the Golgi complex.

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Experimental validation of predicted endocytosis functions in human.

Volume 15, Issue 3, Pages (September 2008)

Role of adaptor-binding motifs in export and segregation of endolysosomal proteins at the Golgi complex. Role of adaptor-binding motifs in export and segregation.

Segregation of endolysosomal proteins in the Golgi complex.

The luminal and transmembrane domains of endolysosomal proteins determine their intra-Golgi segregation. The luminal and transmembrane domains of endolysosomal.

Ralf Jacob, Hassan Y. Naim Current Biology

Structure, localization, and ER exit of RUSH reporter proteins.

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

Mitosis-Resistant Adhesions Provide Molecular Memory to Dividing Cells

Volume 22, Issue 14, Pages (July 2012)

Inhibition of ROCK activity rescues formation of polarized cysts in the absence of DDR1 signaling. Inhibition of ROCK activity rescues formation of polarized.

Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells.

Imaging intracellular endosymbiont E. coli by fluorescent microscopy.

Numerical analysis of cell morphology of tapetal cells in wild type.

Growth factor‐stimulated ERK kinase activities in the cytosol and nucleus are kinetically ordered and do not show dramatic adaptation Growth factor‐stimulated.

Occurrence of prominin-1-containing particles in the ventricular fluid of the embryonic mouse brain. Occurrence of prominin-1-containing particles in the.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Subcellular distribution of TACE-eGFP changes with the coexpression of active ERK2. Subcellular distribution of TACE-eGFP changes with the coexpression.

Volume 26, Issue 4, Pages e6 (April 2019)

Aggresome formation of STAT5A_ΔE18.

Conformational Activation of Argonaute by Distinct yet Coordinated Actions of the Hsp70 and Hsp90 Chaperone Systems Kotaro Tsuboyama, Hisashi Tadakuma,

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Ezrin in its open, activated conformation binds and colocalizes with MISP at the cell cortex. Ezrin in its open, activated conformation binds and colocalizes.

Two approaches for improved live-cell imaging at sub-100-nm resolution

Subcellular localization of prestin and mutant proteins in type I, II and III cells. Subcellular localization of prestin and mutant proteins in type I,

DHA restores peroxisome morphogenesis in AOx- and D-BP-defective fibroblasts. DHA restores peroxisome morphogenesis in AOx- and D-BP-defective fibroblasts.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Adenylyl Cyclase Localization Regulates Streaming during Chemotaxis

DHA induces the formation of Pex11pβ-rich regions on elongated peroxisomes. DHA induces the formation of Pex11pβ-rich regions on elongated peroxisomes.

Activation of glial cells.

Fig. 1. Expression of a Ror-eGFP fusion protein under control of the endogenous Ror promoter in Drosophila embryos. Expression of a Ror-eGFP fusion protein.

Association of NM-HA and NM-GFP with SGs is transient.

PtdIns(4,5)P2 dependence of TbEpsinR and TbCALM membrane targeting.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Ub-binding ability of ERα.

Subnuclear localization of HA-tagged EBNA1 protein.

Detection and classification of dendritic spines in transfected hippocampal neurons. Detection and classification of dendritic spines in transfected hippocampal.

Live-Cell Imaging of Dual-Labeled Golgi Stacks in Tobacco BY-2 Cells Reveals Similar Behaviors for Different Cisternae during Movement and Brefeldin A.

Sox11's partner proteins and targets in differentiated neurons.

Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment Spinning disk confocal microscopy images showing spindle region magnifications.

TSA-mediated changes in cell cycle inhibitor proteins.

Thy1S promoter drives sparse expression of hrGFP in marmoset cortex.

Selective delivery of pIC/PPHAffibody decreases the survival of HER2-overexpressing cells. Selective delivery of pIC/PPHAffibody decreases the survival.

Ectopic expression of CA RSK1 mutant protects melanoma cells from PD98059-mediated apoptosis. Ectopic expression of CA RSK1 mutant protects melanoma cells.

Representative macrophage staining images only from the knee joints of CAIA mice injected s.c. with GalNAc–MASP-3–siRNA duplex 2 or GalNAc–luciferase–siRNA.

Purified Vpr induces double-strand breaks in vitro.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Fig. 1 Differential anterograde transport of CCR5 and TNF.

The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.

Primary GBM cells are permissive to HCMV in vitro.

Presentation transcript:

Endolysosomal proteins are exported from the Golgi complex in two distinct populations of transport carriers. Endolysosomal proteins are exported from the Golgi complex in two distinct populations of transport carriers. (a–c) HeLa cells coexpressing streptavidin–KDEL with each of the indicated reporter proteins were treated with biotin and imaged live by spinning-disk confocal microscopy. The left panel shows single frames captured at the indicated times after addition of biotin. The right panel shows magnified images of the boxed areas. (d–f) HeLa cells coexpressing streptavidin–KDEL with combinations of the indicated reporter constructs were analyzed as in a–c. The left columns show single frames captured at the indicated times after addition of biotin (from Video 2 in d and Video 3 in e). The right columns show magnified images of the boxed areas. Bars: (low magnification) 5 µm; (high magnification) 1 µm. Yu Chen et al. J Cell Biol 2017;216:4141-4151 This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.