Volume 21, Issue 3, Pages 231-235 (September 2014) Enhanced recovery, enrichment and detection of Mycobacterium marinum with the Portable Microbe Enrichment Unit (PMEU)  Elias Hakalehto, Anneli Heitto, Lauri Heitto, Kari Rissanen, Ilkka Pesola, Jouni Pesola  Pathophysiology  Volume 21, Issue 3, Pages 231-235 (September 2014) DOI: 10.1016/j.pathophys.2014.07.005 Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 (a and b) Enhanced mycobacterial enrichment is performed in the PMEU Spectrion® (Samplion Oy, Siilinjärvi, Finland) cultivation and detection unit. The incoming and outgoing gases are sterile filtered. The gases are produced specifically for laboratory use. Bacterial concentrations are indicated by IR, UV or optical light sensors, which measure the change in the amount of transmitted light. Pathophysiology 2014 21, 231-235DOI: (10.1016/j.pathophys.2014.07.005) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 (a and b) Growth curves on M. marinum from PMEU Spectrion® analysis. The culture with gas flow above (a), and the reference culture without gas flow below (b). In the former one, the onset of growth was recorded in less than 12h. Pathophysiology 2014 21, 231-235DOI: (10.1016/j.pathophys.2014.07.005) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Growth of Mycobacterium marinum ATCC 927 [8]. Values for the colony forming units (cfu) after 5 days of cultivation in the PMEU Spectrion® and subsequent plating on M7H9 broth medium with 1.25% agar. Cultures 1 and 2 in enrichment syringes represented growth with gas flow, and cultures 3 and 4 were grown without gas flow. This figure illustrates the situation with full-grown cultures. The resulting higher cell densities in the PMEU cultures with gas bubbles are in line with the observations with the microaerobic Campylobacter sp. [10]. Pathophysiology 2014 21, 231-235DOI: (10.1016/j.pathophys.2014.07.005) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions