Turnip Yellow Mosaic Virus P69 Interacts with and Suppresses GLK Transcription Factors to Cause Pale-Green Symptoms in Arabidopsis  Fangrui Ni, Liang Wu, Qiang Wang, Jian Hong, Yijun Qi, Xueping Zhou  Molecular Plant  Volume 10, Issue 5, Pages 764-766 (May 2017) DOI: 10.1016/j.molp.2016.12.003 Copyright © 2017 The Author Terms and Conditions

Figure 1 TYMV P69 Protein Interacts with and Suppresses GLK Transcription Factors to Cause Pale-Green Symptoms. (A) Analysis of interactions between P69 and GLK1 and GLK2 by yeast two-hybrid assay. (B) Transgenic plants overexpressing P69-HA (P69-HAox) and the glk1 glk2 mutant plants display a pale-green phenotype. Photos were taken of 4-week-old plants. Scale bar, 5 mm. (C) Confocal micrographs of chloroplasts in mesophyll cells from the wild-type Col-0, Col-0 overexpressing P69-HA (P69-HAox), glk1 glk2, and glk1 glk2 complemented with GLK1-Flag. Scale bar, 10 μm. (D) Quantitative RT–PCR analysis of representative downregulated genes in both P69-HAox plants and glk1 glk2 mutant plants. Error bars represent the SD from the mean of three biological replicates; asterisks indicate significant differences between Col-0 and the indicated plants (t test, *P < 0.05). (E) Analysis of the effect of P69 on the transcription activities of GLKs. GUN4::Luc, CHLC::Luc, and HEMA1::Luc reporter constructs and 35::GLK1-Flag were transfected into glk1 glk2 protoplasts with 35S::P69HA (P69) or 35S::GUS (Control). Transcription activities were measured by the dual luciferase reporter assay. Error bars represent the SD from the mean of three biological replicates; asterisks indicate significant differences (t test, *P < 0.05). (F) ChIP–PCR results showing the effect of P69 overexpression on GLK1 binding to the promoters of representative photosynthetic genes. Molecular Plant 2017 10, 764-766DOI: (10.1016/j.molp.2016.12.003) Copyright © 2017 The Author Terms and Conditions