Proteomics analysis of NaPi-IIa C terminus binding to PDZ proteins.

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Proteomics analysis of NaPi-IIa C terminus binding to PDZ proteins. Proteomics analysis of NaPi-IIa C terminus binding to PDZ proteins. Arrays of 95 related class I PDZ domains from various proteins were spotted on nylon membranes and overlaid with the GST-fused C terminus of NaPi-IIa (A) or its truncation thereof for the very last amino acids, TRL (B). Interactions were visualized by a horseradish peroxidase-linked antibody directed against the GST moiety as described previously (41). The PDZ domains spotted in each bin are listed in Table I. Serge M. Gisler et al. Mol Cell Proteomics 2008;7:1362-1377 © 2008 The American Society for Biochemistry and Molecular Biology