Ganglioside GQ1b enhances anti–double-stranded DNA antibody and IgG production of PBMCs from patients with systemic lupus erythematosus Naoko Kanda,

Slides:

Advertisements

Similar presentations

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Advertisements

Kiyoshi Yamazaki, MD, Joseph A. Murray, MD, Hirohito Kita, MD

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen–specific IgE production by stimulation of IL-12 production in mice Shinji.

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G

Akos Heinemann, MD, Gunter J. Sturm, MD, Martina Ofner, BSc, Eva M

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Production of chemokines and proinflammatory and antiinflammatory cytokines by human alveolar macrophages activated by IgE receptors Philippe Gosset,

The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells Shelby P. Umland,

Role of IL-9 in the pathophysiology of allergic diseases

CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgE Ke Zhang, MD, PhD, Ling.

Estrogen enhances immunoglobulin production by human PBMCs

Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ Naoko Kanda, Shinichi.

Differential control of TH1 versus TH2 cell responses by the combination of low-dose steroids with β2-adrenergic agonists Elena Goleva, PhD, Annegret.

Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis Sai Chan,

Kiyoshi Yamazaki, MD, Joseph A. Murray, MD, Hirohito Kita, MD

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Time-dependent cellular population of textured-surface left ventricular assist devices contributes to the development of a biphasic systemic procoagulant.

17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production Naoko Kanda, Shinichi.

Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice Svetlana P. Chapoval,

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Ketoconazole Suppresses Interleukin-4 plus Anti-CD40-Induced IgE Class Switching in Surface IgE Negative B Cells from Patients with Atopic Dermatitis

Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms Lourdes Mozo, PhDa, Abel Gayo, BSa,

17β-Estradiol Enhances Vascular Endothelial Growth Factor Production and Dihydrotestosterone Antagonizes the Enhancement via the Regulation of Adenylate.

Gangliosides GD1b, GT1b, and GQ1b Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production: the Inhibition of Adenylate Cyclase1

Targeting allergen to FcγRI reveals a novel TH2 regulatory pathway linked to thymic stromal lymphopoietin receptor Kathryn E. Hulse, PhD, Amanda J. Reefer,

17β-Estradiol Enhances the Production of Nerve Growth Factor in THP-1-Derived Macrophages or Peripheral Blood Monocyte-Derived Macrophages Naoko Kanda,

Histamine Enhances the Production of Granulocyte-Macrophage Colony-Stimulating Factor via Protein Kinase Cα and Extracellular Signal-Regulated Kinase.

Histamine Inhibits the Production of Interferon-induced Protein of 10 kDa in Human Squamous Cell Carcinoma and Melanoma Naoko Kanda, Shinichi Watanabe

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

The effects of glucocorticoids on human eosinophils

Cyclooxygenase-2 Inhibitor Enhances Whereas Prostaglandin E2Inhibits the Production of Interferon-Induced Protein of 10 kDa in Epidermoid Carcinoma A431

17β-estradiol Inhibits the Production of RANTES in Human Keratinocytes

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Anti-Mycotics Suppress Interleukin-4 and Interleukin-5 Production in Anti-CD3 Plus Anti- CD28-Stimulated T Cells from Patients with Atopic Dermatitis

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

17β-Estradiol Inhibits Oxidative Stress-Induced Apoptosis in Keratinocytes by Promoting Bcl-2 Expression Naoko Kanda, Shinichi Watanabe Journal of Investigative.

Ganglioside GQ1b enhances Ig production by human PBMCs

High Calcium, ATP, and Poly(I:C) Augment the Immune Response to β-Glucan in Normal Human Epidermal Keratinocytes Carren Sy Hau, Yayoi Tada, Sayaka Shibata,

The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma James L. Lordan,

Antigen-specific and nonspecific determinants of cytokine production during topical sensitization of mice to chemical allergens Artin Moussavi, PhDa,

Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms Toby J. Williams, Catherine A. Jones,

P38 Mitogen-activated protein kinase–induced glucocorticoid receptor phosphorylation reduces its activity: Role in steroid-insensitive asthma Elvis Irusen,

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

David M. Essayan, MD, Charity C

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

Larry L. Thomas, PhD, Michelle D. Haskell, Emmanuel U

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Inhibition of protein kinases A and C demonstrates dual modes of response in human eosinophils stimulated with platelet-activating factor Takumi Takizawa,

Qianli Zhuang, MD, PhD, Bruce Mazer, MD

Gp120- and TNF-α–induced modulation of human B cell function: Proliferation, cyclic AMP generation, Ig production, and B-cell receptor expression Christina.

Sulfonamide-reactive lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions Richard S. Kalish, MD,

Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy Esther C. de Jong, MSca, b, Steven Spanhaak,

Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients Pierre Pochard, PhDab, Philippe Gosset, PhDb, Corinne.

TH2 Cytokine-enhanced and TGF-β-enhanced vascular endothelial growth factor production by cultured human airway smooth muscle cells is attenuated by IFN-γ.

Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells Richard K.S.

Julie Negri, BA, S. Brandon Early, BA, John W

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

Keiji Miyazawa, MSc, Akio Mori, MD, PhD, Hirokazu Okudaira, MD, PhD

Intracellular 3′,5′-Adenosine Cyclic Monophosphate Level Regulates House Dust Mite- Induced Interleukin-13 Production by T Cells from Mite-Sensitive Patients.

Presentation transcript:

Ganglioside GQ1b enhances anti–double-stranded DNA antibody and IgG production of PBMCs from patients with systemic lupus erythematosus Naoko Kanda, MD, PhD, Shinichi Watanabe, MD, PhD Journal of Allergy and Clinical Immunology Volume 105, Issue 3, Pages 532-540 (March 2000) DOI: 10.1067/mai.2000.104253 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 Dose dependency for the effect of GQ1b on the antibody production by PBMCs from patients with SLE who were seropositive for anti-dsDNA antibody. PBMCs from an active or inactive patient were cultured with GQ1b for 5 days and were assayed for IgG anti-dsDNA antibody and total IgG production. Values are the means ± SD of triplicate cultures. The dashed line indicates the cutoff point for the presence or absence of anti-dsDNA. Data represent 6 separate experiments with PBMCs from 3 active patients and 3 inactive patients. Analysis of data was performed with 1-way ANOVA with Dunnet’s multiple comparison test (*P < .01 versus control levels). Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 Stimulatory effect of GQ1b on antibody production by PBMCs from 12 active and 8 inactive patients with SLE who were seropositive for anti-dsDNA and 6 active and 4 inactive patients with SLE who were seronegative for this antibody. PBMCs were cultured in the presence (+) or absence (–) of GQ1b (10 μmol/L) for 5 days and were assayed for anti-dsDNA (A) and total IgG (B) production. Each point represents the mean amount of triplicate cultures from individual donors. The solid lines show the group means. The dashed lines indicate the cutoff point for the presence or absence of anti-dsDNA. Analysis of data was performed with the paired t test. Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 Inhibition of the GQ1b effect by various antibodies. PBMCs from active patients with SLE who were seropositive for anti-dsDNA were cultured with (+) or without (–) GQ1b (10 μmol/L) in the presence or absence of various antibodies (each 10 μg/mL) for 5 days and were assayed for anti-dsDNA (A) and total IgG (B) production. The concentration of antibodies was determined as recommended by the manufacturers. Values are means ± SD (n = 6; 3 with CNS lupus and 3 without). *P < .001 versus controls with medium alone. †P < .001 versus cultures with GQ1b alone. Analysis of data was performed with 1-way ANOVA with Scheffe’s multiple comparison test. Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 4 Dose dependency of the GQ1b effect on IL-6 (A) and IL-10 (B) production. T cells, monocytes, and CD5+ or CD5– B cells from an active patient with SLE who was seropositive for anti-dsDNA were cultured with indicated doses of GQ1b for 2 days and were assayed for cytokine production. Values are the means ± SD of triplicate cultures. Analysis of data was performed with 1-way ANOVA with Dunnet’s multiple comparison test (*P < .05 versus control levels). Data represent 6 separate experiments with peripheral blood cells from 6 active patients with SLE who were seropositive for anti-dsDNA (3 with CNS lupus and 3 without). Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 5 IL-6 and IL-10 amounts in the supernatant from GQ1b- or medium-treated T cells (A) and the effects of the supernatants on B-cell antibody production (B) . T cells (1 × 106 cells/mL) from 6 active patients with SLE who were seropositive for anti-dsDNA were cultured with or without GQ1b (10 μmol/L) for 2 days. The culture supernatants were harvested, and 200-μL aliquots were assayed for IL-6 and IL-10. One hundred fifty-microliter aliquots of the supernatants were added to CD5+ or CD5– B cells (2 × 105 cells in 50 μL of fresh medium) from the identical donors, and the B cell fractions were cultured in triplicate. The identical B-cell fractions were simultaneously cultured with medium alone or medium containing GQ1b (7.5 μmol/L). The B-cell antibody production was evaluated after 5 days. Values are means ± SD (n = 6; 3 with CNS lupus and 3 without). Analysis of data in A was performed with the paired t test, and analysis of data in B was performed with the 1-way ANOVA with Scheffe’s multiple comparison test (*P < .05 versus control cultures with medium alone; †P < .05 versus cultures with the supernatant from medium-treated T cells). Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 6 The effects of signal transduction inhibitors on GQ1b-induced IL-6 and IL-10 production. T cells (2 × 105/200 μL/well) from 6 active patients with SLE who were seropositive for anti-dsDNA were preincubated for 1 hour with medium alone or with medium containing signal transduction inhibitors before the addition of GQ1b (10 μmol/L). After 2 days, the culture supernatants were assayed for IL-6 (A) and IL-10 (B) . The inhibitors were staurosporine (STS ; final 1.5 nmol/L), calphostin C (Cal C ; 0.1 μmol/L), H-89 (0.1 μmol/L), herbimycin A (HerA , 1 μmol/L), and calbidazolium chloride (R24571 , 1 μmol/L). The concentration of each inhibitor was determined to inhibit the aimed signal specifically as recommended by the manufacturer. Values are means ± SD (n = 6; 3 with CNS lupus and 3 without). Analysis of data was performed with the 1-way ANOVA with Scheffe’s multiple comparison test (*P < .001 versus control cultures with medium alone; †P < .001 versus cultures with GQ1b alone). Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 7 A schematic diagram for putative immunologic pathways activated by GQ1b. Ab, Antibody. Journal of Allergy and Clinical Immunology 2000 105, 532-540DOI: (10.1067/mai.2000.104253) Copyright © 2000 Mosby, Inc. Terms and Conditions