Ex vivo transfection of pulmonary artery segments in lung isografts

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Presentation transcript:

Ex vivo transfection of pulmonary artery segments in lung isografts Motoki Yano, MD, Masafumi Hiratsuka, MD, Itaru Nagahiro, MD, Bassem N Mora, MD, Ronald K Scheule, PhD, G.Alexander Patterson, MD The Annals of Thoracic Surgery Volume 68, Issue 5, Pages 1805-1809 (November 1999) DOI: 10.1016/S0003-4975(99)00719-5

Fig 1 Chloramphenicol acetyl transferase (CAT) activity in effect-of-exposure-time assessment groups. (A) Gross transgene expression was detected in all groups. (B) Density of gene expression. The density of the 60 minute-exposure group (group 3) was established as the standard (100%). All pulmonary artery segments showed high levels of CAT activity irrespective of exposure time. There were no significant differences between groups (N.S.). The Annals of Thoracic Surgery 1999 68, 1805-1809DOI: (10.1016/S0003-4975(99)00719-5)

Fig 2 Chloramphenicol acetyl transferase activity in effect-of-temperature assessment groups. The group exposed at 10°C (group 5) was established as the standard. Significant and similar levels of transgene expression were detected irrespective of exposure temperature. There were no significant differences between groups (N.S.). The Annals of Thoracic Surgery 1999 68, 1805-1809DOI: (10.1016/S0003-4975(99)00719-5)

Fig 3 Chloramphenicol acetyl transferase activity in effect-of-pressure assessment groups. There were no significant differences between groups (N.S.). The density of both groups was approximately three times lower than the standard group with three times higher lipid-gene construct. The Annals of Thoracic Surgery 1999 68, 1805-1809DOI: (10.1016/S0003-4975(99)00719-5)

Fig 4 Chloramphenicol acetyl transferase activity at the various sacrifice times. Three hours after reperfusion, faint transgene expression was apparent. Gradually the density increased, and 24 hours to 3 days after reperfusion, the expression was maximal. High expression continued for 1 week, and then transgene expression became faint. Four weeks to 8 weeks postoperatively, individual differences were noted, but transgene expression was still apparent. The Annals of Thoracic Surgery 1999 68, 1805-1809DOI: (10.1016/S0003-4975(99)00719-5)

Fig 5 Arterial oxygen tension in effect-of-temperature assessment and effect-of-pressure assessment groups. There were no differences between corresponding groups. The Annals of Thoracic Surgery 1999 68, 1805-1809DOI: (10.1016/S0003-4975(99)00719-5)