Volume 15, Issue 7, Pages 1288-1296 (July 2007) Antisense Oligonucleotide-induced Exon Skipping Across the Human Dystrophin Gene Transcript  Steve D Wilton, Abbie M Fall, Penny L Harding, Graham McClorey, Catherine Coleman, Susan Fletcher  Molecular Therapy  Volume 15, Issue 7, Pages 1288-1296 (July 2007) DOI: 10.1038/sj.mt.6300095 Copyright © 2007 The American Society of Gene Therapy Terms and Conditions

Figure 1 AOs to induce removal of dystrophin exons 2–78. The relative size and annealing position of the AO and the intron and exon lengths are indicated. Molecular Therapy 2007 15, 1288-1296DOI: (10.1038/sj.mt.6300095) Copyright © 2007 The American Society of Gene Therapy Terms and Conditions

Figure 2 Representative examples of AO-induced dystrophin exon skipping for each class of exon. (a) Type 1 exons are efficiently induced at low AO concentration. (b) Type 2 exons are removed to a lesser extent. (c) Type 3 exons are excluded only at high AO concentrations. (d) Type 4 exons require more than one AO for efficient exclusion or a flanking exon(s) is removed in addition to the target exon. Molecular Therapy 2007 15, 1288-1296DOI: (10.1038/sj.mt.6300095) Copyright © 2007 The American Society of Gene Therapy Terms and Conditions