Singularity in Polarization: Rewiring Yeast Cells to Make Two Buds

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Singularity in Polarization: Rewiring Yeast Cells to Make Two Buds Audrey S. Howell, Natasha S. Savage, Sam A. Johnson, Indrani Bose, Allison W. Wagner, Trevin R. Zyla, H. Frederik Nijhout, Michael C. Reed, Andrew B. Goryachev, Daniel J. Lew  Cell  Volume 139, Issue 4, Pages 731-743 (November 2009) DOI: 10.1016/j.cell.2009.10.024 Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 1 Rewiring the Yeast Polarization Feedback Loop (A) Diffusion-mediated amplification (Kozubowski et al., 2008). The panels represent sequential snapshots of the plasma membrane as seen from the cell interior. GTP-Cdc42p arising stochastically at random sites may recruit a GEF-PAK complex from the cytoplasm through direct binding of the PAK (a Cdc42p effector). The associated GEF then promotes GTP-loading of Cdc42p in the immediate vicinity, causing growth of a GTP-Cdc42p cluster. (B) Actin-mediated amplification (Wedlich-Soldner et al., 2003). (1) A membrane protein able to promote actin cable attachment may (2) capture (or nucleate) an actin cable, which then delivers vesicles containing more of the protein toward that site on the plasma membrane, growing a cluster. (3) Slow diffusion and balanced endocytosis maintains the polarized state (Marco et al., 2007). (C) Synthetic Bem1p-GFP-Snc2p protein used to rewire amplification. Oval, Bem1p; green oval, Bem1p-GFP; green oval with a purple tail, Bem1p-GFP-Snc2p; red star, mutations in the Snc2p endocytosis motif. (D) FRAP analysis of diploids homozygous for BEM1-GFP-SNC2 or BEM1-GFP. Average intensities plotted relative to prebleach signal. Recovery half-times are indicated (mean ± SD). Inset: same data, expanded timescale. (E) Bem1p-GFP-Snc2p, but not the endocytosis-deficient Bem1p-GFP-Snc2pV39A,M42A, concentrates at polarization sites (arrows). The scale bar represents 5 μm. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 2 Bem1p-GFP-Snc2p Promotes Polarization and Proliferation of rsr1Δ Cells (A) Tetrads from sporulation of rsr1Δ/rsr1Δ strains heterozygous for bem1Δ, BEM1-GFP, BEM1-GFP-SNC2, or BEM1-GFP-SNC2V39A,M42A as indicated. (B) Bem1p-GFP, Bem1p-GFP-Snc2p and Bem1p-GFP-Snc2pV39A,M42A are expressed at similar levels. Blot probed with anti-GFP and anti-Cdc11p (loading control). (C) BEM1-GFP-SNC2 rsr1Δ cells display polarized Bem1p-GFP-Snc2p. The scale bar represents 5 μm. (D) Wild-type and rewired cells polarize comparable amounts of GTP-Cdc42p, assessed with a PBD-RFP probe (mean ± SEM, n = 14). Inset: examples of live cells. (E) Wild-type and rewired cells displayed a similar cell-cycle profile. All cells in the figure are rsr1Δ. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 3 Bem1p-GFP-Snc2p Biases Polarization toward the Previous Division Site, but Can Also Break Symmetry (A) Budding of BEM1-GFP-SNC2 rsr1Δ haploids is biased toward the previous division site. First-time mothers stained to label the cell wall and birth scar (bright patch, which marks previous division site). (B) Budding of RSR1/RSR1 BEM1/BEM1-GFP-SNC2 diploids is biased toward the previous division site. Multiple-time mothers stained to label bud scars (location of previous division sites). ∗ indicates birth scars, when not obscured by bud scars. (C) Budding of BEM1-GFP-SNC2/BEM1-GFP-SNC2 diploids can occur directly into the birth scar. (D) After centrifugal elutriation (which lengthens G1 in the first cycle), BEM1-GFP-SNC2 rsr1Δ cells break symmetry. In the second cell cycle, budding was again biased. Cells were fixed and stained at 80 min (first cycle) and 180 min (second cycle) after elutriation. (E) Bud site position for first- and second-time mothers from (D). n > 100. Scale bars represent 5 μm in all panels. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 4 Comparison of Actin-Mediated and Diffusion-Mediated Polarization (A) Polarization in rewired cells is actin-dependent. Polarization of PBD-RFP and either Bem1p-GFP or Bem1p-GFP-Snc2p was scored after complete actin depolymerization (200 μM Lat-A, 2 hr). n > 90. (B) Lat-A treated rewired cells do not polarize PBD-RFP, Spa2p-RFP, or Cdc3p-RFP. Montages of live-cell images are shown. The scale bar represents 5 μm. (C) Dynamics of Bem1p-GFP-Snc2p and actin patch polarization (frames from Movie S1). Actin patches visualized with Abp1p-mCherry. Arrows: times of 50% of peak Bem1p-GFP-Snc2p polarization (green), actin patch polarization (red), and time of bud emergence (black; scored from DIC images). The time is in minutes. (D) Quantitation of polarization in (C). Integrated GFP or RFP intensity in the focus is plotted as % of peak intensity for that cell. (E) Dynamics of Bem1p-GFP and actin patch polarization (frames from Movie S2). (F) Quantitation of polarization in (E). (G) Interval between polarization of Bem1p-GFP or Bem1p-GFP-Snc2p and actin patches, scored from times when integrated GFP and RFP intensities reached 50% of peak. Line indicates average. ∗ indicates that the difference is statistically significant (p < 0.001, Student's t test). (H) Interval from first detection of polarized GFP signal to bud emergence. Because polarization of Bem1p-GFP is more abrupt than that of Bem1p-GFP-Snc2p, use of the “50% of peak” criterion for GFP polarization would lead to a bigger difference than the “first detection” criterion used here. ∗ indicates that the difference is statistically significant (p < 0.001, Student's t test). (I) Interval from actin patch polarization (50% of peak) to bud emergence. The difference is not statistically significant (p = 0.14). All cells in the figure are rsr1Δ. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 5 Rewired Cells Violate Singularity (A) Rewired cells can grow two buds simultaneously. BEM1-GFP-SNC2 cells (frames from Movie S3). Time is in hours:minutes. The scale bar represents 2 μm. (B) Comparison of bud growth in side by side one- versus two-bud cells, measured from DIC images (e.g., Movie S4). Open symbols, one-bud cell; closed symbols, two-bud cell. Left, haploid cells. Right, diploid cells. (C) Actin cables and patches are polarized toward both buds. Cells were fixed and stained with rhodamine-phalloidin. The scale bar represents 5 μm. (D) Montages of two-bud cells containing BEM1-GFP-SNC2 (lower panels) and either ABP1-mCherry, CDC3-mCherry, PBD-RFP, or SPA2-mCherry (upper panels). The scale bar represents 5 μm. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 6 Competition between Foci in Rewired Cells (A) Rewired BEM1-GFP-SNC2 rsr1Δ cells formed two foci but then one focus grew while the other disappeared prior to budding; selected frames from Movies S5 and S6. Time is in minutes. The scale bar represents 2 μm. (B) Integrated GFP intensity in each focus for three illustrative BEM1-GFP-SNC2 rsr1Δ cells. Foci from the same cell are colored in dark versus light red, blue, or green. (C) Actin cables are directed toward both foci. Selected Z planes of representative one-focus and two-focus BEM1-GFP-SNC2 rsr1Δ cells fixed and imaged as in Figure 5C. Tracing shows cables that could be unambiguously assigned to a (color-coded) focus. Grey, no clear attachment. (D) Model for Bem1p-GFP-Snc2p dynamics in cells with two foci. Red arrows, vesicular trafficking along actin cables; black arrows, endocytosis; green arrows, diffusion in the plane of the membrane. (E) Effect of focus geometry on diffusion-mediated escape of Bem1p-GFP-Snc2p. Circles represent a top-down view of the cylinders illustrating distribution of Bem1p-GFP-Snc2p (green dots). (F) Simulation of competition between foci with η = 0.5. Bem1p-GFP-Snc2p content of foci from the same cell are colored in dark versus light red, blue, or green. Simulations started with the indicated ratios of protein. (G) Dependence of the competition time on the initial ratio and η. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 7 Competition between Foci in Wild-Type Cells and Cells Overexpressing Bem1p (A) Two-focus (arrows) intermediates in wild-type cells resolve within 1.5 min. Deconvolved images of Bem1p-GFP. Arrowhead, neck. Time is in minutes. The scale bar represents 2 μm. (B) Model for competition between foci in wild-type cells. A small limiting pool of Bem1p-GEF-PAK complex diffuses rapidly in the cytoplasm and can bind GTP-Cdc42p in either focus. GTP-Cdc42p is concentrated in each focus, and can bind the complex or hydrolyze GTP. GDP-Cdc42p can bind to GDI and be extracted from the membrane or can be re-loaded with GTP by neighboring GEFs. (C) Simulation of competition between foci. Bem1p content of foci from the same cell are colored in dark versus light red, blue, or green. Simulations started with the indicated ratios of protein. (D) Dependence of the competition time on the initial ratio and the abundance of the Bem1p-GEF-PAK complex. (E) Cells overexpressing Bem1p display competition between foci. SPA2-mCherry cells containing a high-copy 2 μm-BEM1-GFP plasmid were imaged. Spa2p-mCherry (polarity marker)/DIC overlays shown (selected frames from Movie S7). Time is in minutes. The scale bar represents 2 μm. (F) Quantitation of competition times (interval between first detection of two foci and first detection of a single winning focus). Comparison of rewired (BEM1-GFP-SNC2 rsr1Δ) cells and Bem1p overexpressors. (G) Bem1p overexpressors can violate singularity and make two buds: selected frames from Movie S8. The scale bar represents 2 μm. Cell 2009 139, 731-743DOI: (10.1016/j.cell.2009.10.024) Copyright © 2009 Elsevier Inc. Terms and Conditions