A Comprehensive Analysis of Pattern Recognition Receptors in Normal and Inflamed Human Epidermis: Upregulation of Dectin-1 in Psoriasis Heleen D. de.

Slides:

Advertisements

Similar presentations

Bart Tummers, Renske Goedemans, Veena Jha, Craig Meyers, Cornelis J. M

Advertisements

CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens Junpei Furuta, Takashi Inozume,

Interleukin-10 Downregulates Anti-Microbial Peptide Expression in Atopic Dermatitis Michael D. Howell, Natalija Novak, Thomas Bieber, Saveria Pastore,

Interferon-Gamma Enhances TLR3 Expression and Anti-Viral Activity in Keratinocytes A.i. Kajita, Shin Morizane, Tetsuya Takiguchi, Takenobu Yamamoto, Masao.

Cathelicidin Antimicrobial Peptide LL-37 in Psoriasis Enables Keratinocyte Reactivity against TLR9 Ligands Shin Morizane, Kenshi Yamasaki, Beda Mühleisen,

Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop Florina Olaru,

A Toll-Like Receptor 7, 8, and 9 Antagonist Inhibits Th1 and Th17 Responses and Inflammasome Activation in a Model of IL-23-Induced Psoriasis Weiwen.

EGFR and IL-1 Signaling Synergistically Promote Keratinocyte Antimicrobial Defenses in a Differentiation-Dependent Manner Andrew Johnston, Johann E.

The IL-17A-Producing CD8+ T-Cell Population in Psoriatic Lesional Skin Comprises Mucosa-Associated Invariant T Cells and Conventional T Cells Marcel.

Trim32 Deficiency Enhances Th2 Immunity and Predisposes to Features of Atopic Dermatitis Yuangang Liu, Zhiping Wang, Rachel De La Torre, Ashley Barling,

Thorbjørn Krejsgaard, Ulrik Ralfkiaer, Erik Clasen-Linde, Karsten W

Hanna Niehues, Lam C. Tsoi, Danique A. van der Krieken, Patrick A. M

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis Rahul Purwar, Thomas Werfel, Miriam.

Cytokine Effects Induced by the Human Autoallergen α-NAC

Receptor Activator of NF-κB Ligand Promotes the Production of CCL17 from RANK+ M2 Macrophages Taku Fujimura, Yumi Kambayashi, Sadanori Furudate, Masayuki.

IL-25 Enhances HSV-1 Replication by Inhibiting Filaggrin Expression, and Acts Synergistically with Th2 Cytokines to Enhance HSV-1 Replication Byung Eui.

Preclinical Studies of a Specific PPARγ Modulator in the Control of Skin Inflammation Arianna Mastrofrancesco, Daniela Kovacs, Massimiliano Sarra, Emanuela.

Volume 5, Issue 4, Pages (April 2009)

Increased Sensitivity to Interferon-α in Psoriatic T Cells

Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D George W. Agak, Min Qin, Jennifer Nobe,

Effective Treatment of Psoriasis with Narrow-Band UVB Phototherapy Is Linked to Suppression of the IFN and Th17 Pathways Emőke Rácz, Errol P. Prens,

EGFR Regulates the Expression of Keratinocyte-Derived Granulocyte/Macrophage Colony-Stimulating Factor In Vitro and In Vivo Francesca Mascia, Christophe.

Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes Maria Gschwandtner, Shaomin Zhong,

Pseudomonas Aeruginosa- and IL-1β-Mediated Induction of Human β-Defensin-2 in Keratinocytes Is Controlled by NF-κB and AP-1 Kai Wehkamp, Lars Schwichtenberg,

Regulation of IL-33 Expression by IFN-γ and Tumor Necrosis Factor-α in Normal Human Epidermal Keratinocytes Jitlada Meephansan, Hidetoshi Tsuda, Mayumi.

Crosstalk between Keratinocytes and T Cells in a 3D Microenvironment: A Model to Study Inflammatory Skin Diseases Ellen H. van den Bogaard, Geuranne.

Cytokine-Induced CEACAM1 Expression on Keratinocytes Is Characteristic for Psoriatic Skin and Contributes to a Prolonged Lifespan of Neutrophils Massilva.

The Antimicrobial Heterodimer S100A8/S100A9 (Calprotectin) Is Upregulated by Bacterial Flagellin in Human Epidermal Keratinocytes Arby Abtin, Leopold.

The Antimicrobial Protein Psoriasin (S100A7) Is Upregulated in Atopic Dermatitis and after Experimental Skin Barrier Disruption Regine Gläser, Ulf Meyer-Hoffert,

Stimulation of Purinergic Receptors Modulates Chemokine Expression in Human Keratinocytes Saveria Pastore, Francesca Mascia, Sara Gulinelli, Sylvia Forchap,

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

The Human Skin–Associated Autoantigen α-NAC Activates Monocytes and Dendritic Cells via TLR-2 and Primes an IL-12-Dependent Th1 Response Susanne Hradetzky,

IL-27 Suppresses Antimicrobial Activity in Human Leprosy

Increased Expression of Carbonic Anhydrase II (CA II) in Lesional Skin of Atopic Dermatitis: Regulation by Th2 Cytokines Marijke Kamsteeg, Patrick L.J.M.

S100A15, an Antimicrobial Protein of the Skin: Regulation by E

Vitamin D Analog Calcipotriol Suppresses the Th17 Cytokine–Induced Proinflammatory S100 “Alarmins” Psoriasin (S100A7) and Koebnerisin (S100A15) in Psoriasis

Potential Synergy between SNP and CpG-A or IL-1β in Regulating Transcriptional Activity of IL-20 Promoter Lanqi Wang, Kejia Li, Qiannan Xu, Xiaoying.

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

High Expression Levels of Keratinocyte Antimicrobial Proteins in Psoriasis Compared with Atopic Dermatitis Gys J. de Jongh, Patrick L.J.M. Zeeuwen, Martina.

Keratinocyte-Specific Deletion of the Receptor RAGE Modulates the Kinetics of Skin Inflammation In Vivo Julia S. Leibold, Astrid Riehl, Jan Hettinger,

Inter-Regulation of Th17 Cytokines and the IL-36 Cytokines In Vitro and In Vivo: Implications in Psoriasis Pathogenesis Yijun Carrier, Hak-Ling Ma, Hilda.

Cystatin M/E Expression is Restricted to Differentiated Epidermal Keratinocytes and Sweat Glands: a New Skin-Specific Proteinase Inhibitor that is a Target.

Ekatherina Vassina, Martin Leverkus, Shida Yousefi, Lasse R

Caspase-5 Expression Is Upregulated in Lesional Psoriatic Skin

UVB and Proinflammatory Cytokines Synergistically Activate TNF-α Production in Keratinocytes through Enhanced Gene Transcription Muhammad M. Bashir,

Differential Gene Induction of Human β-Defensins (hBD-1, -2, -3, and -4) in Keratinocytes Is Inhibited by Retinoic Acid Jürgen Harder, Ulf Meyer-Hoffert,

The Development and Characterization of an In Vitro Model of Psoriasis

Hanna Niehues, PhD, Berenice Rösler, MSc, Danique A

Th2 Cytokines Suppress Lipoteichoic Acid–Induced Matrix Metalloproteinase Expression and Keratinocyte Migration in Response to Wounding Anne M. Brauweiler,

Valrubicin in a Topical Formulation Treats Psoriasis in a Xenograft Transplantation Model Cecilia Rosada, Karin Stenderup, Elisabeth de Darkó, Frederik.

Genetics of Psoriasis: Evidence for Epistatic Interaction between Skin Barrier Abnormalities and Immune Deviation Judith G.M. Bergboer, Patrick L.J.M.

IFN-α Enhances IL-22 Receptor Expression in Keratinocytes: A Possible Role in the Development of Psoriasis Mikiko Tohyama, Lujun Yang, Yasushi Hanakawa,

CXCR4 in Epidermal Keratinocytes: Crosstalk within the Skin

Patrick L. J. M. Zeeuwen, Gys J. de Jongh, Ivonne M. J. J

Pimecrolimus Enhances TLR2/6-Induced Expression of Antimicrobial Peptides in Keratinocytes Amanda S. Büchau, Jürgen Schauber, Thomas Hultsch, Anton Stuetz,

Epidermal Expression of Host Response Genes upon Skin Barrier Disruption in Normal Skin and Uninvolved Skin of Psoriasis and Atopic Dermatitis Patients

High Calcium, ATP, and Poly(I:C) Augment the Immune Response to β-Glucan in Normal Human Epidermal Keratinocytes Carren Sy Hau, Yayoi Tada, Sayaka Shibata,

Sensitization via Healthy Skin Programs Th2 Responses in Individuals with Atopic Dermatitis Louise Newell, Marta E. Polak, Jay Perera, Charlotte Owen,

IL-17A Upregulates Keratin 17 Expression in Keratinocytes through STAT1- and STAT3- Dependent Mechanisms Xiaowei Shi, Liang Jin, Erle Dang, Ting Chang,

Transgenic Expression of Interleukin-13 in the Skin Induces a Pruritic Dermatitis and Skin Remodeling Tao Zheng, Min H. Oh, Sun Y. Oh, John T. Schroeder,

Activation of TLR3 in Keratinocytes Increases Expression of Genes Involved in Formation of the Epidermis, Lipid Accumulation, and Epidermal Organelles

Blazej Zbytek, Andrzej T. Slominski

Myeloid Differentiation Factor 88 Regulates Basal and UV-Induced Expressions of IL-6 and MMP-1 in Human Epidermal Keratinocytes Youngae Lee, Hyunjung.

Aldo-Keto Reductase 1C3 Is Expressed in Differentiated Human Epidermis, Affects Keratinocyte Differentiation, and Is Upregulated in Atopic Dermatitis

Distinct Strains of Propionibacterium acnes Induce Selective Human β-Defensin-2 and Interleukin-8 Expression in Human Keratinocytes Through Toll-Like.

Redistribution of LRIG Proteins in Psoriasis

Induction of Matrix Metalloproteinase-9 in Keratinocytes by Histamine

Regulation of IL-13 Receptors in Human Keratinocytes

Possible Pathogenic Role of Th17 Cells for Atopic Dermatitis

Gram-positive anaerobe cocci are underrepresented in the microbiome of filaggrin- deficient human skin Patrick L.J.M. Zeeuwen, PhD, Thomas H.A. Ederveen,

Presentation transcript:

A Comprehensive Analysis of Pattern Recognition Receptors in Normal and Inflamed Human Epidermis: Upregulation of Dectin-1 in Psoriasis Heleen D. de Koning, Diana Rodijk-Olthuis, Ivonne M.J.J. van Vlijmen-Willems, Leo A.B. Joosten, Mihai G. Netea, Joost Schalkwijk, Patrick L.J.M. Zeeuwen Journal of Investigative Dermatology Volume 130, Issue 11, Pages 2611-2620 (November 2010) DOI: 10.1038/jid.2010.196 Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Relative mRNA expression levels of PRRs and related signaling molecules in human epidermis. (a) TLRs; (b) NLRs, CLRs, and RLRs; and (c) other PRR-related signaling molecules. mRNA expression data from human epidermal sheets of normal skin (N=5), psoriatic plaques (N=9), and AD lesions (N=6). TLR4 in normal skin was used as a reference value (relative quantity of 1) in calculating the relative quantities of all gene expression levels. It is to be noted that the relative quantity is presented in a log10 scale. Bars indicate mean±SEM. *P-value <0.05 compared with normal skin. AD, atopic dermatitis; NS, normal skin; PS, psoriasis. Journal of Investigative Dermatology 2010 130, 2611-2620DOI: (10.1038/jid.2010.196) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Protein expression of dectin-1, melanoma differentiation-associated gene-5 (MDA5), and ICEBERG in human epidermis. Immunohistochemical staining of normal skin, psoriatic plaques, and atopic dermatitis lesions. Each picture is representative of data from three different individuals. Bar=100μm. Journal of Investigative Dermatology 2010 130, 2611-2620DOI: (10.1038/jid.2010.196) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Relative mRNA expression levels of CLEC7A (dectin-1) in a panel of human tissues. Equal amounts from each tissue were analyzed by qPCR. CLEC7A expression levels in normal epidermis, psoriasis, and atopic dermatitis were taken from Figure 1. Normal epidermis was used as a reference value (relative quantity of 1) in calculating the relative quantities of all gene expression levels. PBMCs, peripheral blood mononuclear cells. Journal of Investigative Dermatology 2010 130, 2611-2620DOI: (10.1038/jid.2010.196) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Cytokine-mediated induction of CLEC7A (dectin-1) transcription in cultured primary keratinocytes. Confluent cell cultures (N=4–8) were stimulated for 24 and 48hours with IL-4 (50ngml−1) and IL-13 (50ngml−1); IL-17 (30ngml−1) and IL-22 (30ngml−1); IFN-α (500Uml−1); or IFN-γ (500Uml−1). *P-value <0.05, **P-value <0.001. Journal of Investigative Dermatology 2010 130, 2611-2620DOI: (10.1038/jid.2010.196) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Host response gene expression in cultured primary keratinocytes on stimulation with dectin-1, TLR2, and TLR5 ligands. After 24-hour preincubation with IFN-γ, confluent keratinocyte cultures (N=4) were stimulated for 48hours with β-glucan (50μgml−1) or heat-killed (HK) Candida (5*106ml−1) with or without addition of Pam3Cys-SKKKK (10μgml−1) or flagellin (1μgml−1). Gray versus white bars indicate the (synergistic) effect of dectin-1 stimulation and black versus white bars the (synergistic) effect of concerted fungal receptor stimulation. (Supplementary Figure S2 online shows 24-hour stimulation data.) (a) Relative quantity (RQ) of mRNA levels. (b) Protein levels of IL-6, IL-8, and hBD-2 in the supernatants are measured by ELISAs. It is to be noted that the data are presented in a log10 scale. Bars indicate mean±SEM. *P-value <0.05. Journal of Investigative Dermatology 2010 130, 2611-2620DOI: (10.1038/jid.2010.196) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions