Comparative microbiome analysis of Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Tyrophagus putrescentiae Jinyoung Lee, BS, Ju Yeong.

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Presentation transcript:

Comparative microbiome analysis of Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Tyrophagus putrescentiae Jinyoung Lee, BS, Ju Yeong Kim, MD, Myung-hee Yi, PhD, Younjee Hwang, MS, In-Yong Lee, PhD, Sung-Hyun Nam, BS, Dongeun Yong, MD, PhD, Tai-Soon Yong, MD, PhD Journal of Allergy and Clinical Immunology Volume 143, Issue 4, Pages 1620-1623 (April 2019) DOI: 10.1016/j.jaci.2018.10.062 Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Microbiomes of D farinae, D pteronyssinus, and T putrescentiae. A, Pie charts based on species-level classifications. The size of each pie chart is indicative of the read counts, and organisms are indicated only when they accounted for more than 1% of the total in D farinae and T putrescentiae. Percentages of bacteria and read counts accounting for the majority are indicated in the slices, and the total read counts are indicated in the middle of the chart. The microbiome of each species was calculated as the average of 5 different batches. B, Relative 16S rRNA levels in D farinae and D pteronyssinus. Each bar shows the average rRNA level from bacteria in 5 different batches (n = 5 per group). **P < .01. C, Heatmap and dendrogram for mite microbiomes. The rows in the dendrogram indicate hierarchical clustering of the 3 species of mites, D farinae (Df), D pteronyssinus (Dp), and T putrescentiae (Tp), and the columns indicate clustering of species that occurred more often together. D, Concentration of endotoxin in 1 mg/mL of mite body extract (EU/mL). Each bar represents the average of 5 different batches of each species of mites (n = 5 per group). *P < .05, **P < .01. Journal of Allergy and Clinical Immunology 2019 143, 1620-1623DOI: (10.1016/j.jaci.2018.10.062) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Change in the D farinae (D.f.) microbiome following antibiotics treatment and its effects on the allergic inflammatory response. A, Relative 16S rRNA levels. B, Concentration of endotoxin in 1 mg/mL of D farinae extract (EU/mL). C, Concentration of Der f 1 in 1 mg/mL of D farinae extract (ng/mL). D, Concentrations of IL-6 and IL-8 of human bronchial epithelial cells (BEAS2-B) treated with the extract of D farinae cultured under ampicillin (AMP) (pg/mL). Each bar shows the average of different batches (n = 4 per group). *P < .05, **P < .01. Journal of Allergy and Clinical Immunology 2019 143, 1620-1623DOI: (10.1016/j.jaci.2018.10.062) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Journal of Allergy and Clinical Immunology 2019 143, 1620-1623DOI: (10.1016/j.jaci.2018.10.062) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Journal of Allergy and Clinical Immunology 2019 143, 1620-1623DOI: (10.1016/j.jaci.2018.10.062) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Journal of Allergy and Clinical Immunology 2019 143, 1620-1623DOI: (10.1016/j.jaci.2018.10.062) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions