BT_0370 galactokinase and BT_371 glucose/galactose transporter

Slides:

Advertisements

Similar presentations

III. Linkage A. ‘Complete’ Linkage B. ‘Incomplete’ Linkage C. Three-point Mapping - combine complementary sets Three Point Test Cross AaBbCc x aabbcc Phenotypic.

Advertisements

Research Papers.. Biology 423L Research Paper: Genetics behind cloning of a human gene: Outline due Oct. 23.

Background Gregory Fischer Julie Anderson Daniel Herman  Department of Biology  University of Wisconsin-Eau Claire Heterologous expression of MBP1 from.

DNA LIBRARIES Dr. E. What Are DNA Libraries? A DNA library is a collection of DNA fragments that have been cloned into a plasmid and the plasmid is transformed.

Molecular Biology II Lecture 1 OrR. Restriction Endonuclease (sticky end)

BIODEEP MEETING - Marseille, April 2002 Workpackages 4 and 5 - Fabienne MOREL, Sam DUKAN Objectives : isolation of new strains from DHABs Interface.

Chapter 5 Sequence Assembly: Assembling the Human Genome.

Molecular Genetic Analysis and Biotechnology

CRISPR/Cas9 screening using unique molecular identifiers

GFP‐Sed5p localization defect in sgt2Δ.

Growth rate‐dependent gene expression under glucose limitation.

Nur77 interacts with COUP‐TF in yeast.

Vav‐1 gene‐targeting strategy.

RESULTS AND DISCUSSION

Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.

Principles of using neural networks for predicting molecular traits from DNA sequence Principles of using neural networks for predicting molecular traits.

Schematic diagram of the three circuits analysed in this work.

CRISPR knockdown of the mrdAB operon increases cell width at high repression strengths CRISPR knockdown of the mrdAB operon increases cell width at high.

Overview of the experimental setting.

Hnf4g importance in human colon cancer organoids and regulation of the Hnf4a locus Hnf4g importance in human colon cancer organoids and regulation of the.

(A) Outline of the malignant transformation process.

Sensitivity of RNA‐seq.

Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.

Structure of in‐frame deletions.

Functional evaluation of ATR1 and PHO84 genes located at ePTL13.

Confirmation of pool data with isogenic culture

Large‐scale image‐based CRISPR‐Cas9 gene perturbation profiling

Silencing in Yeast rDNA Chromatin

Expression of PD‐1 on PBMC and malignant exudate T cells

Heat maps showing global relative growth phenotype and comparison between measured and predicted values. Heat maps showing global relative growth phenotype.

Targeted Complementation of MHC Class II Deficiency by Intrathymic Delivery of Recombinant Adenoviruses Ronald Rooke, Caroline Waltzinger, Christophe.

The transcript profiles in the three human cell lines based on RNA sequencing (RNA‐seq). The transcript profiles in the three human cell lines based on.

Western blots validate ClpXP‐mediated degradation of FtsZ in vivo during starvation and synthesis of FtsZ with glucose pulsing Western blots validate ClpXP‐mediated.

Splenic CD169+ macrophages express a unique gene profile.

Feature‐based COG enrichment analysis

Volume 16, Issue 8, Pages (August 2016)

Sensor optimization for thiosulfate and tetrathionate detection in the gut Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A.

Examples for intrachromosomal mRNA co‐regulation patches

Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+

Mean feature profiles of targeted cells are highly consistent between gRNA sequences Mean feature profiles of targeted cells are highly consistent between.

Bisection mapping of T7. RNAP

Characterization of Factor 5 (oxidative stress response factor) in the CLL data Characterization of Factor 5 (oxidative stress response factor) in the.

Patient organoids respond more diverse to drugs and with lower therapeutic potential than 2D cultured patient cells Patient organoids respond more diverse.

Optimality principles in adaptive evolution.

CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk CRISPR knockdown can be multiplexed to modulate expression of.

Volume 86, Issue 1, Pages (July 1996)

Recursive construction and error correction of a simple combinatorial library. Recursive construction and error correction of a simple combinatorial library.

QRT–PCR analysis of tissue‐specific candidate gene expression in response to reduced IIS qRT–PCR analysis of tissue‐specific candidate gene expression.

Building and using sequence‐expression‐activity maps (SEAMAPs) AThe RBS Library Calculator designs a synthetic RBS library to efficiently search a multi‐dimensional.

Two‐way unsupervised uncentered unnormalized hierarchical clustering using a Pearson's correlation of the phenotypic profiles of 4281 nonessential genes.

Transcriptomic and epigenetic changes associated with Factor 1 in the scMT data Transcriptomic and epigenetic changes associated with Factor 1 in the scMT.

Comparison of proteomics and RNA‐Seq data.

DominoEffect R package

Transformation of expression data to identify more direct consequences of perturbation Expression changes under amino acid starvation conditions (30 min,

An integrated NHR network.

Antisense‐mediated regulation of SUR7.

TIPI mediates rapid degradation of proteins in yeast.

Combining substitutions at sites I–V gives rise to signal response, bifunctional behavior, and robustness. Combining substitutions at sites I–V gives rise.

Global and focused views of human interaction map.

Schematics for nutrient pulse systems

Rare and abundant codons.

Schematic of the inverse PCR cloning strategy and growth analysis of the CRISPRi library. Related to Fig 2 Schematic of the inverse PCR cloning strategy.

Characterization results of the lysis device in the final system using 3OC12HSL. Characterization results of the lysis device in the final system using.

(A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). (A) Observed.

DuMPLING oligonucleotide plasmid library design and production

Mouse Y1H pipeline. Mouse Y1H pipeline. (A) Workflow to retrieve the mouse TF open‐reading frame (ORF) clone resource. We predict that the mouse genome.

CRISPR‐Cas9 gene perturbation profiling in HeLa cells

Validation of select PDN drug candidates in an in vivo endotoxemia model Validation of select PDN drug candidates in an in vivo endotoxemia model Therapeutic.

Figure Genetic characterization of the novel GYG1 gene mutation (A) GYG1_cDNA sequence and position of primers used. Genetic characterization of the novel.

Presentation transcript:

BT_0370 galactokinase and BT_371 glucose/galactose transporter BT_0370 galactokinase and BT_371 glucose/galactose transporter Selection kinetics by fragments per kilobase mapped (FPKM) fold change and normalized effective coverage of genes BT_0368, BT_0369, BT_0370, BT_0371, and BT_0372.Mapped reads to each base in the region with deep sequencing and Sanger sequencing of isolated clones (below). Read values are the mean across five mice and were normalized to 1 billion mapped bases per run to compare across time points. Isolation of individual clones allowed for insert size profiling at Day 7. Screened isolates from Day 28 did not reveal any galactokinase inserts.Functional characterization in minimal media with galactose as the sole carbon source. Three sets of strains were studied: (i) starting E. coli strains transformed with the CDS of each gene cloned into the backbone vector, (ii) E. coli clones directly isolated from stool samples, and (iii) starting E. coli strains re‐transformed with individual plasmids isolated from stool samples. All clones isolated at Day 28 carried the BT_1759 locus. Lines represent the mean.Genotyping of the background E. coli genome at the galK locus. 20 clones isolated from mice inoculated with the library at each of the indicated time points were screened, while 30 clones isolated from the lux control mice at Day 28 were screened. Source data are available online for this figure. Stephanie J Yaung et al. Mol Syst Biol 2015;11:788 © as stated in the article, figure or figure legend