B. Timothy Baxter, MD, Gregory S. McGee, MD, Vera P

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Presentation transcript:

Elastin content, cross-links, and mRNA in normal and aneurysmal human aorta B.Timothy Baxter, MD, Gregory S. McGee, MD, Vera P. Shively, BS, Iain A.S. Drummond, PhD, Saryu N. Dixit, PhD, Mitsuo Yamauchi, MD, William H. Pearce, MD Journal of Vascular Surgery Volume 16, Issue 2, Pages 192-200 (August 1992) DOI: 10.1016/0741-5214(92)90107-J Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 Algorithm for extraction of elastin from aortic tissue (AA, Amino acid analysis). Journal of Vascular Surgery 1992 16, 192-200DOI: (10.1016/0741-5214(92)90107-J) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Comparison of Northern blot analysis of total RNA from normal (NOR) human infrarenal aorta obtained from transplant donors, infrarenal aorta affected with atherosclerosis (AOD) but no aneurysmal dilation, and infrarenal aorta from patients with abdominal aortic aneurysm (AAA). Total RNA extracted from bovine nuchal ligament (BNL) was used as a positive control for elastin. The nitrocellulose transfers were hybridized with complimentary DNA sequences to elastin (3.5 kb), α-tubulin (1.7 kb) and α1(I) procollagen (4.8 and 5.8 kb). Age and sex of patients are shown at bottom of each lane. Journal of Vascular Surgery 1992 16, 192-200DOI: (10.1016/0741-5214(92)90107-J) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 Chromatogram of hydrolyzed NaOH insoluble residue demonstrates DID peak. Journal of Vascular Surgery 1992 16, 192-200DOI: (10.1016/0741-5214(92)90107-J) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 4 Chromatogram of thermolysin digested NaOH insoluble residue demonstrates the peak for VAPG. Journal of Vascular Surgery 1992 16, 192-200DOI: (10.1016/0741-5214(92)90107-J) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions