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Presentation transcript:

Micropipettes

Micropipette Parts

Micropipette Display Window

Gel Electrophoresis

Molecules Molecules vary in size, shape and charge DNA has a negative charge due to the phosphate groups 10,000 bp DNA piece 250 bp DNA piece 700 bp DNA piece

Gel Electrophoresis Acts as a molecular strainer, separating molecules based upon size, shape and charge Smaller fragments travel faster and farther away from the wells than larger fragments

Gel Electrophoresis

Larger Fragments Smaller Fragments

Agarose Gel Tray

Agarose Gel Tray

Gel Box with Buffer

More About Gel Electrophoresis Two most common gels are agarose and polyacrylamide Buffer: stabilizes pH, maintains molecule shape, and conducts electricity

Gel Stains Ethidium Bromide (EtBr) – glows orange when exposed to UV light Methylene Blue – dark blue bands SYBR® Safe – glows green GelGreen GelRed