Journal of Allergy and Clinical Immunology

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Journal of Allergy and Clinical Immunology Correction Journal of Allergy and Clinical Immunology Volume 101, Issue 6, (June 1998) DOI: 10.1016/S0091-6749(98)70319-7 Copyright © 1998 Terms and Conditions

FIG. 3 IgE responses to salivary allergens of six mosquito species in subjects allergic to mosquitos and nonallergic subjects. Proteins of saliva or salivary gland extracts separated by SDS-PAGE were transferred to nitrocellulose membranes. Membranes were sequentially incubated with sera from subjects allergic to mosquitos (strips 1 to 12) or sera from nonallergic subjects (strips 13 to 17), monoclonal anti-human IgE, and enzyme-conjugated goat anti-mouse IgG as described in Methods section. Infant serum (strip 18),cord serum (strip 19), and PBST (strip 20) were used as negative controls. Six mosquitos species used were Ae. aegypti (A), Ae. vexans (B), Ae. albopictus (C), Ae. togoi (D), Cx. quinquefasciatus (E), and Cx. pipiens (F).In Ae. vexans a 68 kd species-shared allergen, Aed a 1, is indicated by arrow a, and a 23 kd Ae. vexans-specific allergan is indicated by arrow b. Journal of Allergy and Clinical Immunology 1998 101, DOI: (10.1016/S0091-6749(98)70319-7) Copyright © 1998 Terms and Conditions