Theoretical Background Dayun Yu Department of Physics Kangwon National University Surface Plasmon Resonance (SPR) Optical principle. Dispersion relation Advantage of the surface plasmon resonance Mass Transport in Boundary Layer Effect of the mass transport Benefit of the mass transport

= Optical Principle (SPR) : Condition of Resonance Light (ω) angle Reflectivity Light (ω) 2D-detector array Surface Plasmon wave vector Evanescent wave vector Condition of Resonance = :

Dispersion Relation K w w0 Theory of SPR Ksp= w/c x {(emr x es)/(emr + es)}1/2 Ks=w/c x (es)1/2 Kretschmann’s prism method Kev=Kp x sinq=w/c x (ep)1/2 x sinq Kev=Ksp (Resonance point : w0) qsp=sin x [(emx es)/{ep(emr + es)}]1/2 Fig. Dispersion of evanescent wave vector(kev) and surface plasmon wave vector(ksp)

Advantage of the SPR Monitor binding rapidly Measurement in real time Process experiment without labels

Effect of Mass Transport Gold surface Flow Mass transport Sample (analyte) Ligand Diffusion } Binding Binding of analyte onto the gold surface becomes more dominant than the diffusion of analyte near the surface.

Benefit of the Mass Transport Determine the active concentration and flow rate of analyte The investigation of fluidics

Summery Surface Plasmon Resonance - Detection of binding is based on the SPR phenomenon - Advantage of SPR # Monitor binding rapidly # Measurement in real time # Process experiment without labels Mass transport in boundary layer - Mass transport occur that binding of analyte onto the gold surface becomes more dominant than the diffusion of analyte near the surface - Benefit of mass transport # Determine the active concentration and flow rate of the analyte # Investigation of the fluidics