Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I

Slides:

Advertisements

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Advertisements

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages (October 2014) DOI:

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

Graft smooth muscle cells specifically synthesize increased collagen

Clinical evaluation of recombinant human platelet – derived growth factor for the treatment of lower extremity diabetic ulcers David L. Steed, MD, the.

Heparin-coated catheters and heparin-induced thrombocytopenia

Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies? John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.

Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure Oscar.

Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R

Mitogen-activated protein kinase activation: An alternate signaling pathway for sustained vascular smooth muscle contraction Aaron M. Epstein, MD, Douglas.

Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α Alan Dardik,

The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro Carol J. Sawmiller, MD, Richard J. Powell, MD,

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R

A new valvulotome for in situ bypass grafts

Hiroyuki Itoh, MD, Peter R

Angiotensin II-induced growth of vascular smooth muscle cells requires an Src- dependent activation of the epidermal growth factor receptor1 Dirk Bokemeyer,

Richard R. Keen, MD, Kevin D

Modulation of vascular smooth muscle cell alignment by cyclic strain is dependent on reactive oxygen species and P38 mitogen-activated protein kinase

Malcolm O. Perry, MD, Richard Kempczinski, MD

Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis

Platelet-derived growth factor is a cofactor in the induction of 1α(I) procollagen expression by transforming growth factor β1 in smooth muscle cells

Heparin-coated catheters and heparin-induced thrombocytopenia

Cigarette smoke alters chylomicron metabolism in rats

A chemically modified dextran inhibits smooth muscle cell growth in vitro and intimal in stent hyperplasia in vivo Jean-François Deux, MDa,b, Sandrine.

Nitric oxide prevents p21 degradation with the ubiquitin-proteasome pathway in vascular smooth muscle cells Melina R. Kibbe, MD, Suhua Nie, MD, Dai-Wu.

Protein kinase C-δ regulates migration and proliferation of vascular smooth muscle cells through the extracellular signal-regulated kinase 1/2 Bo Liu,

Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies? John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.

Antisense basic fibroblast growth factor alters the time course of mitogen-activated protein kinase in arterialized vein graft remodeling Akimasa Yamashita,

Reduction of hospital resources utilization in vascular surgery: A four-year experience Sean P. Roddy, MD, Thomas F. O'Donnell, MD, Mark D. Iafrati, MD,

Objective tinnitus resulting from internal carotid artery stenosis

George H. Meier, MD, Bauer Sumpio, MD, PhD, Henry R

Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,

Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth.

Smooth muscle cell migration and proliferation are mediated by distinct phases of activation of the intracellular messenger mitogen-activated protein.

The effect of growth factors, cytokines, and extracellular matrix proteins on fibronectin production in human vascular smooth muscle cells Terry L. Kaiura,

Calvin B. Ernst, MD Journal of Vascular Surgery

Thrombin activates MAPKAP2 kinase in vascular smooth muscle

Ruptured abdominal aortic aneurysm: A population-based study

Captopril renal scintigraphy: A new standard for predicting outcome after renal revascularization George Henry Meier, MD, Bauer Sumpio, MD, PhD, John.

Cardiovascular surgery—The rocket and its stars: Presidential address

Composite sequential bypasses to the ankle and beyond for limb salvage

Research and research funding in vascular surgery

Activation of integrin receptors is required for growth factor–induced smooth muscle cell dysfunction Kyotaro Mawatari, MD, Bo Liu, PhD, K.Craig Kent,

Interleukin-1β inhibits PDGF-BB−induced migration by cooperating with PDGF-BB to induce cyclooxygenase-2 expression in baboon aortic smooth muscle cells

J.Dennis Baker, MD Journal of Vascular Surgery

Alex Limanni, M. D. , Timothy Fleming, Ph. D. , Rodolfo Molina, M. D

Volume 56, Issue 6, Pages (December 1999)

Regional nerve block for femoropopliteal and tibial arterial reconstructions Joseph M. Giordano, M.D., George A. Morales, M.D., Hugh H. Trout, M.D., Ralph.

Substitution by adenosine for glucagon as a surgical diagnostic tool to detect mesenteric vascular adaptation István Dóbi, MD, Alexander Juhász-Nagy,

Richard J. Sanders, MD 1, William H. Pearce, MD 2

Should patient age be a consideration in carotid endarterectomy?

The effects of endothelial injury on smooth muscle cell proliferation

Exposure of femoral vessels by a suprainguinal incision

Monitoring vascular surgical performance

Modulation of monocyte adherence to endothelial cells by endothelin-1 involvement of Src (p60src) and JAK1-like kinases Lindsey J. Chisholm, MB, BS,

D. Emerick Szilagyi, MD—An appreciation

Presidential address: The second-generation vascular surgeon

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Fibroblasts cultured from venous ulcers display cellular characteristics of senescence Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD,

Jerry Goldstone, MD Journal of Vascular Surgery

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Popliteal entrapment syndrome: A report of tibial nerve entrapment

Molecular biology: A brief overview

Susan M. Nesselroth, MDa, Alliric I

Cornelius A. Sullivan, MD, Michael J. Rohrer, MD, Bruce S. Cutler, MD

Presentation transcript:

Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I. Willis, MD, George P. Tuszynski, PhD, Bauer E. Sumpio, MD, PhD Journal of Vascular Surgery Volume 29, Issue 6, Pages 1031-1036 (June 1999) DOI: 10.1016/S0741-5214(99)70244-2 Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 1 Growth-arrested confluent vascular smooth muscle cells were treated with thrombospondin-1 (20 μg/mL) for 0, 15, 30, and 120 minutes. Cell lysates were subjected to antiphosphotyrosine (RC20H) immunoblotting. Three prominent bands were identified at 68 kDa, 125 kDa, and 180 kDa. Journal of Vascular Surgery 1999 29, 1031-1036DOI: (10.1016/S0741-5214(99)70244-2) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 2 Growth-arrested vascular smooth muscle cells were treated with thrombospondin-1 (20 μg/mL) for 15, 30, and 120 minutes. Serum-free medium was the negative control. Cell lysates were subjected to immunoprecipitation with anti-focal adhesion kinase antibodies. A, Antiphosphotyrosine immunoblotting demonstrates tyrosine phosphorylation of focal adhesion kinase, hence activation. B, Immunoblotting with anti-focal adhesion kinase antibodies confirms equal loading of protein and no change in focal adhesion kinase levels. Journal of Vascular Surgery 1999 29, 1031-1036DOI: (10.1016/S0741-5214(99)70244-2) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 3 Phosphorylation of focal adhesion kinase quantified by means of densitometry in four different experiments. Normalized data demonstrates enhanced tyrosine phosphorylation of focal adhesion kinase for thrombospondin-1 stimulated vascular smooth muscle cells, compared with cells in serum-free medium (P < .05), and was no different from cells exposed to 10 ng/mL platelet-derived growth factor (positive control). Journal of Vascular Surgery 1999 29, 1031-1036DOI: (10.1016/S0741-5214(99)70244-2) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions