An Egr-1 master switch for arteriogenesis: Studies in Egr-1 homozygous negative and wild-type animals Cristian Sorin Sarateanu, MD, Mauricio A. Retuerto,

Slides:

Advertisements

Similar presentations

Tubular heart valves: A new tissue prosthesis design—Preclinical evaluation of the 3F aortic bioprosthesis James L. Cox, MD, Niv Ad, MD, Keith Myers, BS,

Advertisements

Lost in translation The Journal of Thoracic and Cardiovascular Surgery

Manuel J. Antunes, MD, PhD, DSc

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia Go Kuwahara,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia Geoffrey O. Ouma,

“Triplet” polycistronic vectors encoding Gata4, Mef2c, and Tbx5 enhances postinfarct ventricular functional improvement compared with singlet vectors

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats

The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Gorav Ailawadi, MD, Christopher W. Moehle, BS, Hong Pei, MD, Sandra P

Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps Francesca Polito, PhD, Alessandra Bitto, MD, PhD, Mariarosaria.

Combination of stromal-derived factor-1α and vascular endothelial growth factor gene- modified endothelial progenitor cells is more effective for ischemic.

Ryan M. McEnaney, MD, Ankur Shukla, MD, Michael C

Metformin stimulates ischemia-induced revascularization through an eNOS dependent pathway in the ischemic hindlimb mice model Noriko Takahashi, MD, Rei.

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

The days of future past Neel K. Ranganath, MD, Aubrey C. Galloway, MD

Aging impairs the angiogenic response to ischemic injury and the activity of implanted cells: Combined consequences for cell therapy in older recipients

Intrinsic cardiac stem cells are essential for regeneration

Abracadabra I, II…HeartMate 3?

Form ever follows function

Endothelial nitric oxide synthase affects both early and late collateral arterial adaptation and blood flow recovery after induction of hind limb ischemia.

William M. DeCampli, MD, PhD

How should we treat air leaks?

New treatment approaches create new disease processes: A short guide on how to reduce unexpected events to a minimum Martin Czerny, MD, MBA The Journal.

Salvage angiogenesis induced by adenovirus-mediated gene transfer of vascular endothelial growth factor protects against ischemic vascular occlusion

The lord of the rings Antonio Miceli, MD, PhD

Masakuni Kido, MD, Lingling Du, MD, Christopher C

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Tormod S. Westvik, MD, Tamara N

Loss of CRNN expression is associated with advanced tumor stage and poor survival in patients with esophageal squamous cell carcinoma Po-Kuei Hsu, MD,

The assessment of cost effectiveness and the effectiveness of cost assessment in cardiothoracic surgery Vinay Badhwar, MD The Journal of Thoracic and.

It's not “just a shunt” but sometimes it should be…

Gene therapy with adenovirus-mediated myocardial transfer of vascular endothelial growth factor 121 improves cardiac performance in a pacing model of.

Military surgeons just want to have fun

Impairment of human cell–based vasculogenesis in rats by hypercholesterolemia- induced endothelial dysfunction and rescue with l-arginine supplementation

Do we need a bibliometrician to know which way the wind is blowing?

A first start for lung transplantation?

Angiogenic pretreatment to enhance myocardial function after cellular cardiomyoplasty with skeletal myoblasts Mauricio A. Retuerto, BS, James T. Beckmann,

Joseph A. Dearani, MD, Michael J. Ackerman, MD, PhD

A fusion protein of hepatocyte growth factor enhances reconstruction of myocardium in a cardiac patch derived from porcine urinary bladder matrix Takeyoshi.

Restoration of left ventricular geometry and improvement of left ventricular function in a rodent model of chronic ischemic cardiomyopathy Jiashing Yu,

Controlled delivery of vascular endothelial growth factor promotes neovascularization and maintains limb function in a rabbit model of ischemia Steven.

The Ross procedure: Time to reevaluate the guidelines

Manuel J. Antunes, MD, PhD, DSc

The Journal of Thoracic and Cardiovascular Surgery

Passing the torch The Journal of Thoracic and Cardiovascular Surgery

Prognosis and “granularity”: Building on staging foundations?

Patrick T. Roughneen, MD, Grant T. Fankhauser, MD, Abe DeAnda, MD

Functional tricuspid pathology: To treat or not to treat

Macrophage depletion reduces monocyte chemotactic protein-1 and transforming growth factor-β1 in healing rat vein grafts Randal A Wolff, PhD, Jeffrey.

Overexpression of endothelial nitric oxide synthase increases skeletal muscle blood flow and oxygenation in severe rat hind limb ischemia Lucy S Brevetti,

The Journal of Thoracic and Cardiovascular Surgery

Discussion The Journal of Thoracic and Cardiovascular Surgery

Tissue valve, nitinol stent, or storage solution

The future of cardiac surgery training: A survival guide

Should it stay or should it go?

Vinay Badhwar, MD, John S. Ikonomidis, MD, PhD, Jeffrey P. Jacobs, MD

“The more things change…”: The challenges ahead

Commentary: Judgment day: Should you add atrial fibrillation ablation?

Why arch curvature affects arch resistance

Toward a more rational approach in treating type B aortic dissection

Stromal cell-derived factor and granulocyte-monocyte colony-stimulating factor form a combined neovasculogenic therapy for ischemic cardiomyopathy Y.

Respect the aorta The Journal of Thoracic and Cardiovascular Surgery

The evolution of cardiothoracic critical care

Therapeutic angiogenesis in patients with severe limb ischemia by transplantation of a combination stem cell product Gabriel P. Lasala, MD, FACC, Jose.

Who should decide margin length in pulmonary excision of lung cancer?

Did you like Terminator 3 better than Terminator 2

Samuel Kim, MD The Journal of Thoracic and Cardiovascular Surgery

Journal changes and initiatives

Presentation transcript:

An Egr-1 master switch for arteriogenesis: Studies in Egr-1 homozygous negative and wild-type animals Cristian Sorin Sarateanu, MD, Mauricio A. Retuerto, BS, James T. Beckmann, BS, Leslie McGregor, BS, JoAnn Carbray, BS, Gerald Patejunas, PhD, Lina Nayak, Jeffrey Milbrandt, MD, Todd K. Rosengart, MD The Journal of Thoracic and Cardiovascular Surgery Volume 131, Issue 1, Pages 138-145 (January 2006) DOI: 10.1016/j.jtcvs.2005.08.052 Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Quantitative analysis of perfusion index, calculated as described in the Methods section. Note near normalization (index approximately 1) of perfusion in AdEgr-treated animals at day 21. AdEgr-1, Adenoviral vector encoding for Egr-1; PBS, phosphate-buffered saline; AdNull, noncoding vector. The Journal of Thoracic and Cardiovascular Surgery 2006 131, 138-145DOI: (10.1016/j.jtcvs.2005.08.052) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Laser Doppler perfusion imaging at days 7 and 21 after excision. Low or no blood perfusion is displayed as dark blue, and the highest perfusion level is displayed as red. Note early reconstitution of flow in AdEgr-1–treated animals and near equalization at day 21. PBS, Phosphate-buffered saline; AdNull, noncoding vector; AdEgr-1, adenoviral vector encoding for Egr-1. The Journal of Thoracic and Cardiovascular Surgery 2006 131, 138-145DOI: (10.1016/j.jtcvs.2005.08.052) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Top, Representative lesions as seen 21 days after excision: a, healed pressure sores, soft tissue loss trace without scab (2+); b, nonhealed pressure sores, partial thickness and skin loss covered with scab (3+); c, necrosis of the tips of the toes (4+); and d, gangrene with amputation of phalanges-toes (5+). Please see the Methods section for details. Bottom, Distribution of lesions between groups. AdEgr, adenoviral vector encoding for Egr. The Journal of Thoracic and Cardiovascular Surgery 2006 131, 138-145DOI: (10.1016/j.jtcvs.2005.08.052) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Density of arterioles and capillaries in ligated rat hind limb 21 days after specified treatments, as identified with smooth muscle cell actin (A) and lectin stains (B), respectively. SMA, Smooth muscle actin; Egr, early growth response gene (Ad transfer). The Journal of Thoracic and Cardiovascular Surgery 2006 131, 138-145DOI: (10.1016/j.jtcvs.2005.08.052) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Photomicrographs of arterioles and capillaries in ligated rat hind limbs after specified treatments, as identified by smooth muscle cell actin (A) and lectin stains (L), respectively. Top panels, adenoviral vector encoding for Egr-1 (AdEgr); middle panels, phosphate-buffered saline (PBS); bottom panels, noncoding vectors (AdNull). The Journal of Thoracic and Cardiovascular Surgery 2006 131, 138-145DOI: (10.1016/j.jtcvs.2005.08.052) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions