Three-dimensional structure and IgE-binding properties of mature fully active Der p 1, a clinically relevant major allergen Sonia de Halleux, MSc, Enrico.

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Presentation transcript:

Three-dimensional structure and IgE-binding properties of mature fully active Der p 1, a clinically relevant major allergen Sonia de Halleux, MSc, Enrico Stura, PhD, Luc VanderElst, MSc, Vincent Carlier, MSc, Marc Jacquemin, MD, PhD, Jean-Marie Saint- Remy, MD, PhD Journal of Allergy and Clinical Immunology Volume 117, Issue 3, Pages 571-576 (March 2006) DOI: 10.1016/j.jaci.2005.11.032 Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 Purification and characterization of recombinant mature Der p 1–N52Q. A, Denaturing electrophoresis. Left, Molecular weight standards; right, 2 μg purified protein. B, HPLC analysis of 50 μg Der p 1. C, Hydrolysis of Boc-Gln-Ala-Arg-AMC by 3.5 μg purified protein. RFU, Relative fluorescence units. Journal of Allergy and Clinical Immunology 2006 117, 571-576DOI: (10.1016/j.jaci.2005.11.032) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 Allergenicity of recombinant mature Der p 1. A, ImmunoCAP binding: IgE quantification. B, Inhibition ELISA. Allergen concentrations are in μg/mL. C, Correlation between percentage of inhibition by rDer p 1 and natural Der p 1 in D pteronyssinus extract, tested at equimolar concentrations. D, Basotests on 4 Der p 1–sensitized patients. P1, rDer p 1; DPT, D pteronyssinus extract. Journal of Allergy and Clinical Immunology 2006 117, 571-576DOI: (10.1016/j.jaci.2005.11.032) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 Ribbon diagrams of mature Der p 1. A, View of the Der p 1 dimer. B, Catalytic site of Der p 1. C, Network of coordinating residues to the magnesium ion. D, Localization of B-cell epitopes as described.10,11 Same view as in A but at a 90° angle. E, Localization of amino acid substitutions in Der p 1 isoforms. Journal of Allergy and Clinical Immunology 2006 117, 571-576DOI: (10.1016/j.jaci.2005.11.032) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions