DNA Isolation 1. Blend for seconds: 1 cup of broccoli

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Presentation transcript:

DNA Isolation 1. Blend for 15-30 seconds: 1 cup of broccoli 1/8 teaspoon salt 1 cup cold water Pour liquid through sieve 3. Add, stir gently, and let sit for 10 min: 2 tablespoons detergent 1/8 teaspoon meat tenderizer Pour off small amount into tube and gently add equal volume isopropanol to form a layer. DNA is visible where layers meet

DNA Isolation 1. Blend for 15-30 seconds: 1 cup of broccoli 1/8 teaspoon salt 1 cup cold water

DNA Isolation 1. Blend for 15-30 seconds: 1 cup of broccoli 1/8 teaspoon salt 1 cup cold water Pour liquid through sieve

DNA Isolation 1. Blend for 15-30 seconds: 1 cup of broccoli 1/8 teaspoon salt 1 cup cold water Pour liquid through sieve 3. Add, stir gently, and let sit for 10 min: 2 tablespoons detergent 1/8 teaspoon meat tenderizer

DNA Isolation 1. Blend for 15-30 seconds: 1 cup of broccoli 1/8 teaspoon salt 1 cup cold water Pour liquid through sieve 3. Add, stir gently, and let sit for 10 min: 2 tablespoons detergent 1/8 teaspoon meat tenderizer Pour off small amount into tube and gently add equal volume isopropanol to form a layer. DNA is visible where layers meet