GARLIC & HEALTH WP2: breeding Embryogenesis Half year meeting Munich

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Presentation transcript:

GARLIC & HEALTH WP2: breeding Embryogenesis Half year meeting Munich 30 sept.-2 oct. 2003 WP2: breeding Embryogenesis

Objectives Embryogenesis WP2: breeding Setting a somatic embryogenesis procedure Comparative characterisation of plants from somatic embryo-genesis and from classic micro-propagation after meristem culture (as control)

Work plan Establishment of somatic embryogenesis process for : Rouge de La Réunion, Messidrôme, Morasol & Printanor Production and provision of embryogenic cell suspension cultures of these varieties Genetic evaluation of somatic embryo derived plants through flow cytometry (P8), biochemistry (P5), molecular markers (P1), morphological and physiological analyses (P8, P9)

Varietal effect on somatic embryogenesis from cell suspension culture

Deliverables DP 5: Evidence of a somatic embryogenesis process and plant regeneration and acclimatisation in garlic (Allium sativum L.) L. FEREOL, V. CHOVELON, S. CAUSSE, N. MICHAUX FERRIERE and R. KAHANE, 2002. Plant Cell Reports 21: 197-203 DP13: Establishment of garlic (Allium sativum L.) cell suspensions and plant regeneration L. FEREOL, V. CHOVELON, S. CAUSSE, D. TRIAIRE, N. MICHAUX FERRIERE and R. KAHANE Submitted to Plant Science in June 2003 DP21: paper on the use of somatic embryogenesis for rapid multiplication of garlic in vitro. In progress (to submit to In Vitro Cell. Dev. Biol.)

What will be done till 2004 Paper DP 21 Embryogenic cell suspension cultures as A method for garlic (Allium sativum L.) mass propagation Characterising the embryogenic process : Histological studies: achieved Cytometric studies: running No maintenance of the material Field evaluation : running

CIRAD Embryogenesis WP2: breeding L. Féréol S. Causse M. Roux-Cuvelier somatic embryogenesis, histology and flow cytometry, paper writing S. Causse technical assistant somatic embryogenesis, histology and flow cytometry M. Roux-Cuvelier field trials in La Réunion R. Kahane paper revision, management