A20 E3 ligase mediates RIP1 K63-linked polyubiquitination.

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A20 E3 ligase mediates RIP1 K63-linked polyubiquitination. A20 E3 ligase mediates RIP1 K63-linked polyubiquitination. A, in vitro ubiquitination was performed in a reaction consisting of the components as indicated (top) with ubiquitinated RIP1 (RIP1-Ub) detected by an avidin antibody on immunoblotting. B, in vivo ubiquitination was performed by transfecting HEK293T cells with the vectors encoding Flag-RIP1, A20, and HA-UB mts and detecting RIP1-Ub by immunoblotting using Myc and HA antibody. C, in vivo ubiquitination in U87MG cells were conducted by transfecting the cells with the vectors encoding HA-UB and mts, treating the transfectants with 100 ng/mL TRAIL for 1.5 hours, isolating RIP1 under denaturing condition, and detecting RIP1-Ub by immunoblotting using antibodies to HA and RIP1. The number represents the quantification of the density. D, the preligand assembly complex (PLAC) and DISC were isolated from LN443 cells through immunoprecipitation by the use of Flag-TRAIL and Flag antibody. RIP1-Ub was detected by overexposure of immunoblotting using a RIP1 antibody. E, RIP1 was purified through immunoprecipitation under denaturing conditions from the PLAC and DISC as in (D) and RIP1-Ub was detected by immunoblotting by the use of an antibody for ubiquitin. F, RIP1 isolated from LN443 cells as in (E) was examined by immunoblotting by the use of antibodies specific to K63-linked polyubiquitin chain and RIP1. G, the PLAC and DISC were isolated from LN71 clones expressing A20 wt, OTU, and Znf mt through immunoprecipitation as in (F) and examined by immunoblotting with antibodies specific to K63-linked polyubiquitin chain and RIP1. Anita C. Bellail et al. Cancer Discovery 2012;2:140-155 ©2012 by American Association for Cancer Research