Spatial distribution of Cre-dependent tdTomato-labeled neurons in the medullary reticular formation in VgluT2-tdTomato, Dbx1-tdTomato, and Sst-tdTomato.

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Spatial distribution of Cre-dependent tdTomato-labeled neurons in the medullary reticular formation in VgluT2-tdTomato, Dbx1-tdTomato, and Sst-tdTomato double-Tg mouse strains. Spatial distribution of Cre-dependent tdTomato-labeled neurons in the medullary reticular formation in VgluT2-tdTomato, Dbx1-tdTomato, and Sst-tdTomato double-Tg mouse strains. A–C, Confocal fluorescence microscopy images of parasagittal sections at the level of nucleus ambiguus (NA) and coronal sections at the level of the pre-BötC from adult VgluT2-tdTomato (A), Dbx1-tdTomato (B), and Sst-tdTomato (C) mice, representing an overview of distributions of Cre-dependent tdTomato-labeled neurons (red). Motoneurons of NA and the XII immunostained with ChAT antibody (white) provide reference landmarks for levels of the medulla represented in the sections. The parasagittal sections illustrate the extensive distribution of tdTomato-labeled neurons throughout the ventral medullary reticular formation, including within the pre-BötC, the BötC, and the rVRG. Coronal sections illustrate that tdTomato-labeled neurons are distributed within the pre-BötC, and adjacent regions of the reticular formation (RF), including the intermediate RF dorsomedial to NA, where hypoglossal premotor neurons are located. VS, Ventral surface; d, dorsal; c, caudal. Hidehiko Koizumi et al. eneuro 2016;3:ENEURO.0081-16.2016 ©2016 by Society for Neuroscience