Impact of phosphatase deletion on archaella expression and motility.

Slides:



Advertisements
Similar presentations
2D PAGE/Western blotting analysis for the identification of possible post-translation modification of Hsp27 from the spinal cord tissue of the SCI rat.
Advertisements

Correlation of log-transformed signal intensity from two Affymetrix microarray hybridizations using platelet RNA. Plotted are those probesets with an average.
Sequence alignment of C-terminal phosphorylated plant aquaporins
Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,
Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial cells. Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial.
Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.
Differential conjugation of endogenous SUMO-1 and endogenous SUMO-2/3 to target proteins.A and B, SUMO-1 and SUMO-2 proteins were produced in E coli and.
Comparison of protein synthesis patterns of L
Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,
Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three.
Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.
Model of the involvement of phosphorylation in the regulation of motility in S. acidocaldarius. Model of the involvement of phosphorylation in the regulation.
Assay of NOS activity. Assay of NOS activity. Box show total NOS activity, the calcium-dependent activity of the constitutive isoforms of NOS (eNOS and.
Two-dimensional gel silver staining and two-dimensional immunoblotting using antibody to 3-nitrotyrosine. Two-dimensional gel silver staining and two-dimensional.
Expression of apocrine markers at various stages of apocrine carcinoma progression. Expression of apocrine markers at various stages of apocrine carcinoma.
Relative abundance of proteins identified in MALDI IMS
Membrane protein overexpression affects the pH of the culture and protein secretion.A, the pH of the culture of GFP fusion-overexpressing cells and the.
Bottom-up proteomic characterization of MALDI IMS samples.
Success rates in validation of antibodies from external providers
Results from the Morris water task.
Technical reproducibility and biological variability.
Degree of glycosylation of human milk LF from individual donors across lactation. Degree of glycosylation of human milk LF from individual donors across.
A, myelinated nerve fibers in the control and biopsied mouse striatum were stained with anti-MBP antibody. A, myelinated nerve fibers in the control and.
NanoLC-MS/MS/based analysis of proteome differences between colonospheres and isogenic differentiated tumor cells. NanoLC-MS/MS/based analysis of proteome.
The evolutionary conservation of the phosphoproteomes.a, E. coli. b, B. subtilis. The evolutionary conservation of the phosphoproteomes.a, E. coli. b,
BIRC6 is expressed in the tumorigenic Aldefluorhigh fraction of colonosphere cells. BIRC6 is expressed in the tumorigenic Aldefluorhigh fraction of colonosphere.
Colonopshere-enriched proteins display functional interactions.
Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.
High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1.
BIRC6 confers resistance against cisplatin and oxaliplatin.
Cleavage and splicing in a representative specific substrate sequence by yeast active site β subunits. Cleavage and splicing in a representative specific.
A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.
Validation of p53- and miRNA-mediated down-regulation
Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.
Altered pathways in prostate cancer.
Interaction networks of the regulated phosphoproteins.
Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum. Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum.A,
Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated.
Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.
A, Absolute ion intensities of m/z 322, 922 and 1522 as function of the transfer time. A, Absolute ion intensities of m/z 322, 922 and 1522 as function.
Differential expression of apoA-I and Vimentin on 2D gels
K-Means clustering of protein and mRNA expression patterns after PPAR agonists treatments. k-Means clustering of protein and mRNA expression patterns after.
Number of genes/antibodies included in the database.
Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target.
Transcriptionally regulated genes in Δsaci_ptp and Δsaci_pp2a as compared with the strain MW001. Transcriptionally regulated genes in Δsaci_ptp and Δsaci_pp2a.
Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.
Western blot analysis of fetuin-A in AGA and IUGR UC plasma after digestion with glycosidases showing the elimination of the IUGR-related isoforms following.
Localization of selected clones in mammalian COS-7 cells.
Differential expression levels of archaella operon genes and respiratory chain genes in Δsaci_pp2a and Δsaci_ptp. Differential expression levels of archaella.
Biochemical characterization of the protein phosphatases Saci-PP2A.
Biochemical characterization of the protein phosphatases Saci-PTP.
Preferential conjugation of proteins to SUMO-1 or SUMO-2
Voronoi treemaps (109) comparing protein expression profiles of M
Changes in mRNA levels do not correlate with changes in protein levels in upf1Δ and xrn1Δ cells. Changes in mRNA levels do not correlate with changes in.
Illustration of chromatography metric C-2A applied to LC-MS/MS data from three Thermo LTQ systems in analyses of yeast proteome samples in CPTAC Study.
Classification of the 1458 identified proteins into molecular functions. Classification of the 1458 identified proteins into molecular functions. The pie.
Separation of colonospheres from differentiated tumor cells by cluster analysis. Separation of colonospheres from differentiated tumor cells by cluster.
2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.
Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE. Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE.
Expression of σ in SCCs Expression of σ in SCCs. Shown is a magnified section of a representative 2D PAGE gel run with a lysate from an SCC.
Antibody specificity ascertained by 2D-PAGE Western immunoblotting (IEF) of total cellular protein extracts from the RT4 human bladder cancer cell line.
Eight serum proteins (P-selectin, E-selectin, IL-2sRα, IL-18, NE, uPA, uPAR, and CRP) that are elevated in infected neonates function in a molecular network.
Proteomics analysis of NaPi-IIa C terminus binding to PDZ proteins.
Eight serum analytes with greatest differences in levels between clinically infected and non-infected neonates. Eight serum analytes with greatest differences.
SiRNA knockdown of dynein IC2-C recovered the inhibition of neurite outgrowth in NF1-KD PC12 cells. siRNA knockdown of dynein IC2-C recovered the inhibition.
GeneGoTM-based signaling pathway annotations of proteins identified in CD56+ NK cell subsets. GeneGoTM-based signaling pathway annotations of proteins.
Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.
The average median S.D. and PEV reduction after applying different normalization methods compared with raw data. The average median S.D. and PEV reduction.
Fur homodimerization and functional assays
SlRd2 expression confers LiCl resistance in yeast and its dimerization is required for its biological function. SlRd2 expression confers LiCl resistance.
Presentation transcript:

Impact of phosphatase deletion on archaella expression and motility. Impact of phosphatase deletion on archaella expression and motility.A, For the nutrient limitation assay MW001, Δsaci_ptp and Δsaci_pp2a were grown to an OD600 of about 0.4 in Brock medium supplemented with 0.1% NZ-amine, 0.2% sucrose and 10 μg/ml uracil and then transferred to medium lacking the nutrient source. After 6 h samples were collected and immunoblotting was performed with specific antibodies against the archaellum proteins FlaB and FlaX. The amount of protein in Δsaci_ptp is comparable to the strain MW001, whereas for Δsaci_pp2a highly elevated expression of FlaB and FlaX was observed after the starvation stress. Interestingly, already before stress induction the FlaB levels were increased in the Saci-PP2A deletion mutant. B, The wild type strain MW001, the two phosphatase deletion strains Δsaci_ptp and Δsaci_pp2a, the hypermotile positive control strain ΔaapF and the negative nonmotile strain ΔaapFΔflaH were spotted on semi-solid Gelrite dishes and incubated for 5 days at 76 °C. Only a small halo is visible for MW001 and Δsaci_ptp, whereas Δsaci_pp2a was as hypermotile as the positive control strain. Julia Reimann et al. Mol Cell Proteomics 2013;12:3908-3923 © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.