SP3 provides an efficient means for preparing protein and peptide samples for MS analysis Schematic of the SP3 workflow in a single tube. SP3 provides.

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SP3 provides an efficient means for preparing protein and peptide samples for MS analysis Schematic of the SP3 workflow in a single tube. SP3 provides an efficient means for preparing protein and peptide samples for MS analysis Schematic of the SP3 workflow in a single tube. Protein and peptide mixtures are bound to carboxylate‐coated paramagnetic beads through the addition of acetonitrile in a manner similar to HILIC and ERLIC. Immobilization on the bead surface permits rinsing and removal of contaminating substances prior to proteolysis or MS analysis. Elution is performed directly into aqueous solution. Red text indicates steps carried out at the protein level, and blue are performed on peptides. SP3 is compatible with a variety of commonly used reagents. Table of common reagents used in proteomics studies that we have tested and determined to be compatible with SP3. Listed values are the maximum concentrations tested. Reagents that do not appear in this table have not been tested and may be compatible with SP3. SP3 demonstrates high recovery for both proteins and peptides. SDS–PAGE analysis of a yeast whole‐cell lysate left untreated (Control) or treated with SP3. Numerical values at the top of each lane indicate the amount of starting material (μg of protein). Plot on the right displays overlaid densitometry data from the 37.5 μg lanes. Base‐peak chromatograms of equivalent peptide mixtures analyzed by MS after treatment with StageTips (Control) or with SP3. Christopher S Hughes et al. Mol Syst Biol 2014;10:757 © as stated in the article, figure or figure legend