Patrizia Maria Ciotti, B. Sc. , Eleonora Porcu, M. D

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Presentation transcript:

Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing Patrizia Maria Ciotti, B.Sc., Eleonora Porcu, M.D., Leonardo Notarangelo, B.Sc., Otello Magrini, B.Sc., Antonia Bazzocchi, M.D., Stefano Venturoli, M.D. Fertility and Sterility Volume 91, Issue 6, Pages 2399-2407 (June 2009) DOI: 10.1016/j.fertnstert.2008.03.013 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Polscope imaging during slow freezing. Spindle visualization during incubation in freezing solutions (FS2 = freezing solution 2, FS3 = freezing solution 3). Magnification, ×200. Fertility and Sterility 2009 91, 2399-2407DOI: (10.1016/j.fertnstert.2008.03.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Polscope imaging during vitrification. Spindle visualization during incubation in vitrification solutions (WS = wash solution; ES1, ES2, ES3 = equilibration solutions 1, 2, 3; VS = vitrification solution). Magnification, ×200. Fertility and Sterility 2009 91, 2399-2407DOI: (10.1016/j.fertnstert.2008.03.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Polscope imaging during warming. Early spindle visualization during incubation in wash solution 2 (WS2). Magnification, ×200. Fertility and Sterility 2009 91, 2399-2407DOI: (10.1016/j.fertnstert.2008.03.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Spindle cumulative recovery rate during in vitro culture in the four groups: slow freezing; vitrification room temperature (RT)/RT, RT/37°C, and 37°C/37°C. Fertility and Sterility 2009 91, 2399-2407DOI: (10.1016/j.fertnstert.2008.03.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions