Detection of rpoB, katG and inhA gene mutations in Mycobacterium tuberculosis clinical isolates from Chongqing as determined by microarray  C. Yao, T.

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Detection of rpoB, katG and inhA gene mutations in Mycobacterium tuberculosis clinical isolates from Chongqing as determined by microarray  C. Yao, T. Zhu, Y. Li, L. Zhang, B. Zhang, J. Huang, W. Fu  Clinical Microbiology and Infection  Volume 16, Issue 11, Pages 1639-1643 (November 2010) DOI: 10.1111/j.1469-0691.2010.03267.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Schematic diagram of the microarray design. Probes were spotted in quintuplicate. (a) The pattern of oligonucleotides of the rpoB gene printed on the array surface. (b) The pattern of oligonucleotides of the katG and inhA gene printed on the array surface. QC, tetramethyl-6-carboxyrhodamine-labelled oligonucleotide used for quality control of surface chemistry; IC, internal control probe for PCR; EC, external control probe for hybridization-based quantitation; BC, dimethyl sulphoxide control for the quality of microarray; NC, negative control probe; WT, wild-type. Clinical Microbiology and Infection 2010 16, 1639-1643DOI: (10.1111/j.1469-0691.2010.03267.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Examples of DNA microarray hybridization pattern for H37Rv ATCC 27294 and rpoB531 Mct. (a) Representative hybridizations with reference strain. (b) Representative hybridizations with TB clinical isolate rpoB531 Mct. Clinical Microbiology and Infection 2010 16, 1639-1643DOI: (10.1111/j.1469-0691.2010.03267.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

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