13.2 – Manipulating DNA
The Tools of Molecular Biology Genetic engineering – making changes in the DNA code of a living organism DNA extraction – by a simple chemical procedure Cutting DNA – most DNA to large to be analyzed Can be cut precisely at restriction enzymes Separating DNA – gel electrophoresis
Using the DNA Sequence After the DNA is shortened up it can now be read, studied and even changed The function of different genes can now be discovered
Gel Electrophoresis DNA plus restriction enzyme Power source Longer fragments Shorter fragments Mixture of DNA fragments Gel