Different IgE recognition of mite allergen components in asthmatic and nonasthmatic children Yvonne Resch, MSc, Sven Michel, MSc, Michael Kabesch, MD,

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Different IgE recognition of mite allergen components in asthmatic and nonasthmatic children Yvonne Resch, MSc, Sven Michel, MSc, Michael Kabesch, MD, Christian Lupinek, MD, Rudolf Valenta, MD, Susanne Vrtala, PhD Journal of Allergy and Clinical Immunology Volume 136, Issue 4, Pages 1083-1091 (October 2015) DOI: 10.1016/j.jaci.2015.03.024 Copyright © 2015 The Authors Terms and Conditions

Fig 1 IgE reactivity of asthmatic and nonasthmatic children with HDM allergy to individual HDM allergens. A, IgE binding prevalences (y-axis: percentage) to the allergens as measured with ImmunoCAP ISAC technology for nonasthmatic (white bars) and asthmatic (gray bars) children with HDM allergy. Significant differences between the 2 groups are shown. *P < .05 and **P < .01. B, Number of HDM allergens (x-axes) recognized by nonasthmatic (left panel) or asthmatic (right panel) children with HDM allergy (y-axes: percentages of IgE-reactive patients). Gray boxes denote numbers of allergens recognized by 74% and 70% of the nonasthmatic and asthmatic children, respectively. Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig 2 IgE levels to individual HDM allergens. Specific IgE levels (in ISU, y-axis) to HDM allergens (x-axis) determined for reactive children with HDM allergy with (A, right columns) and without (NA, left columns) asthma are displayed as scatter plots. The number of IgE-positive sera for the individual allergens are displayed below. Statistically significant differences between the 2 groups are indicated. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig 3 Frequencies of IgG reactivities to individual HDM allergens in asthmatic and nonasthmatic children with HDM allergy, as well as nonatopic subjects. IgG-binding frequencies (y-axis: percentages) to the allergens (x-axis) are shown for nonatopic (light gray), nonasthmatic (white bars), and asthmatic (dark gray bars) patients with HDM allergy. Statistically significant differences between the groups are indicated. **P < .01. Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig 4 Percentages of sera with certain IgE and IgG reactivity profiles to HDM allergens. Displayed are percentages (y-axes) of sera among the group of asthmatic and nonasthmatic children with HDM allergy containing only IgE (violet), IgE and IgG (green), only IgG (red) or neither IgE nor IgG (blue) to each of the allergens (x-axes). Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig 5 Comparison of allergen-specific IgG levels in nonasthmatic and asthmatic children with HDM allergy containing only allergen-specific IgG or allergen-specific IgE and IgG. Specific IgG levels (y-axes) are displayed for each of the HDM allergens (x-axes) for the nonasthmatic (NA, left columns) and asthmatic (A, right columns) groups. Medians are indicated, and significant differences between only IgG-positive (red diamonds) and IgE- and IgG-positive (green circles) sera are indicated by asterisks. **P < .01. Significant differences between nonasthmatic and asthmatic children in the IgG levels in the only IgG-positive groups (for Der p 1 and Der p 10) are indicated (blue asterisks). *P < .05. Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig 6 Correlations between IgE (x-axes) and IgG (y-axes) antibody levels to individual allergens for nonasthmatic (left panels) and asthmatic (right panels) children with HDM allergy are displayed as scatter plots. IgE- and/or IgG-negative values are depicted as 0.15 ISU (half of the cutoff value) to allow a log-scale presentation of the data. The correlation coefficient ρ and levels of significance (P values) are shown in the lower right corner of the graphs. Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig E1 Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig E2 Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig E3 Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions

Fig E4 Journal of Allergy and Clinical Immunology 2015 136, 1083-1091DOI: (10.1016/j.jaci.2015.03.024) Copyright © 2015 The Authors Terms and Conditions