Mitoprotection as a strategy to prevent chondrocyte death and cartilage degeneration following mechanical injury  M.L. Delco, E.D. Bonnevie, H.H. Szeto, L.J. Bonassar, L.A. Fortier  Osteoarthritis and Cartilage  Volume 24, Pages S500-S501 (April 2016) DOI: 10.1016/j.joca.2016.01.916 Copyright © 2016 Terms and Conditions

Figure 1. SS-31 prevents Impact-induced chondrocyte death Figure 1. SS-31 prevents Impact-induced chondrocyte death. A) Control and (B,C) impacted cartilage stained for live (green)/dead (red) cells and imaged at 24 hr. C) SS-31 treatment 1 hr after injury. D) Cell death in impacted cartilage treated at 0 or 1 hr is equivalent to un-injured controls. Osteoarthritis and Cartilage 2016 24, S500-S501DOI: (10.1016/j.joca.2016.01.916) Copyright © 2016 Terms and Conditions

Figure 2. SS-31 prevents cell membrane damage after cartilage Injury Figure 2. SS-31 prevents cell membrane damage after cartilage Injury. Cartilage explants (n = 18) were impacted then incubated tor 7 days with or without SS-31. LDH activity was measured m cartilage conditioned media every 24 hr. Data is expressed as cumulative LDH activity over the culture period. *p < 0.05. Osteoarthritis and Cartilage 2016 24, S500-S501DOI: (10.1016/j.joca.2016.01.916) Copyright © 2016 Terms and Conditions

Figure 3. SS-31 prevents cartilage matrix degradation after Injury Figure 3. SS-31 prevents cartilage matrix degradation after Injury. Cartilage explants (n = 18) were impacted then incubated with or without SS-31. DMMB assay on cartilage conditioned media revealed A) GAG loss increased in impacted samples by 96 hr post-impact. B) Treatment at 0, 1 or 6 hr after impact decreased GAG loss at 96 hr. *p < 0.05. Osteoarthritis and Cartilage 2016 24, S500-S501DOI: (10.1016/j.joca.2016.01.916) Copyright © 2016 Terms and Conditions