Lipid-binding proteins in rat and human kidney

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Lipid-binding proteins in rat and human kidney Hideki Kimura, M.D., Hiroshi Fujii, Satoru Suzuki, Teruo Ono, Masaaki Arakawa, Fumitake Gejyo  Kidney International  Volume 56, Pages S159-S162 (July 1999) DOI: 10.1046/j.1523-1755.1999.07141.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Immunolight micrographs of human kidney with polyclonal antibody against human heart-type fatty acid-binding protein. (A) In glomeruli, the immunostaining is found in the capillary walls but not in the mesangial area. Frozen sections were not counterstained with hematoxylin and eosin. (B) In interstitium, the staining is observed in the distal tubules. Sections from the paraffin-embedded specimens were counterstained with hematoxylin and eosin. Magnification: (A) ×400; (B) ×200. Kidney International 1999 56, S159-S162DOI: (10.1046/j.1523-1755.1999.07141.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Immunoblot analysis of different tissue homogenates probed with polyclonal antibody against human heart-type fatty acid-binding protein. Several species of human tissues were obtained from autopsy samples from a single patient. Homogenates (30 μg) of the tissues and a blood sample (30 μg) were subjected to immunoblot analysis. Lane 1, isolated glomeruli; lane 2, heart; lane 3, isolated renal tubules; lane 4, blood; lane 5, adrenal tissues; lane 6, small intestine; lane 7, lung; lane 8, spleen. Kidney International 1999 56, S159-S162DOI: (10.1046/j.1523-1755.1999.07141.x) Copyright © 1999 International Society of Nephrology Terms and Conditions