The absence of the centrosome modulates actin filaments organization via FA signaling in a migrating cell. The absence of the centrosome modulates actin.

Slides:

Advertisements

Similar presentations

Fig. S1; Ritter et al Relative induction of p-IRAK1 Relative induction of p-RAF Relative induction of p-MEK1/2 Relative induction of p-Erk1 Relative.

Advertisements

A Role for PML3 in Centrosome Duplication and Genome Stability

Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells. Poly-GR and poly-PR interact with overlapping proteins in.

Protein kinase B (PKB/c-akt) regulates homing of hematopoietic progenitors through modulation of their adhesive and migratory properties by Miranda Buitenhuis,

Cell Physiol Biochem 2013;31: DOI: /

Volume 10, Issue 4, Pages (October 2009)

Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia by Matthias Trost, Martin.

NPM1 drives poly-GR into the nucleolus of primary neurons.

Differential proteome analysis of normal and osteoarthritic chondrocytes reveals distortion of vimentin network in osteoarthritis S. Lambrecht, M.Pharm.,

Volume 22, Issue 17, Pages (September 2012)

Volume 22, Issue 22, Pages (November 2012)

BET inhibition and depletion repress the expression of BRCA1 and RAD51

Analytical Characteristics of Cleavable Isotope-Coded Affinity Tag-LC-Tandem Mass Spectrometry for Quantitative Proteomic Studies Cecily P. Vaughn, David.

Metastatic State of Cancer Cells May Be Indicated by Adhesion Strength

Volume 19, Issue 11, Pages (June 2017)

Alice C.L. Len, Shimona Starling, Maitreyi Shivkumar, Clare Jolly

Validation of astrocyte and microglial proteomic markers that increase across the ALS‐FTD spectrum Validation of astrocyte and microglial proteomic markers.

by Silvia Mele, Stephen Devereux, Andrea G

Volume 7, Issue 3, Pages (September 2016)

HRTV and SFTSV NSs, but not UUKV NSs, inhibits JAK/STAT IFN signaling.

Laura S. Bisogno, Matthew B. Friedersdorf, Jack D. Keene

Protein microarrays for validation of antibodies.

Contribution of Src-FAK signaling to the induction of connective tissue growth factor in renal fibroblasts A. Graness, I. Cicha, M. Goppelt-Struebe

The absence of the centrosome changes global microtubule dynamics in a migrating cell. The absence of the centrosome changes global microtubule dynamics.

T exosomes bind MAdCAM-1 via RA-increased integrin α4β7.

ER stress response and susceptibility to apoptosis are regulated by TFEB and TFE3 ER stress response and susceptibility to apoptosis are regulated by TFEB.

LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

Functional networks of proteins associated with SL3a, SL3abc, SL123, and D3 transcripts. Functional networks of proteins associated with SL3a, SL3abc,

Validation of astrocyte and microglial proteomic markers that increase across the ALS‐FTD spectrum Validation of astrocyte and microglial proteomic markers.

Vimentin is dispensable for multiple B cell–intrinsic processes.

Significant differences in translational efficiencies of DNA damage repair pathway genes between patient clusters. Significant differences in translational.

SIRT1 Is Necessary for Proficient Telomere Elongation and Genomic Stability of Induced Pluripotent Stem Cells Maria Luigia De Bonis, Sagrario Ortega,

Comparison of N. gonorrhoeae gene expression in infected men in vivo and isolates grown in vitro. Comparison of N. gonorrhoeae gene expression in infected.

Regulation of Focal Adhesions by Flightless I Involves Inhibition of Paxillin Phosphorylation via a Rac1-Dependent Pathway Zlatko Kopecki, Geraldine.

The absence of the centrosome promotes small GTPase Rac1 activation via acentrosomal microtubules. The absence of the centrosome promotes small GTPase.

Daple is required for activation of Gαi3, Rac1, and AKT signals and in the antagonistic inhibition of β-catenin–dependent Wnt signals downstream of EGF/EGFR.

Z-FA-FMK stabilized both SMN-FL and SMN-Δ7 proteins in Myc-SMN2a– and Myc-SMN2d–inducible lines. Z-FA-FMK stabilized both SMN-FL and SMN-Δ7 proteins in.

Global Analysis of Palmitoylated Proteins in Toxoplasma gondii

Establishment of intercellular adhesion in homozygous, heterozygous andβ -catenin-null mutant keratinocytes after incubation in medium containing 1.2 mM.

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

Effect of Z-FA-FMK on the expression of SMN proteins in SMA patient iPSCs and iPSC-derived motor neurons. Effect of Z-FA-FMK on the expression of SMN proteins.

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

Extracellular Matrix Rigidity Promotes Invadopodia Activity

Volume 15, Issue 2, Pages (April 2016)

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Reduced Rab11 abundance impairs LFA-1 recycling and consequently LFA-1–dependent migration in T lymphocytes. Reduced Rab11 abundance impairs LFA-1 recycling.

AAS-dependent induction of autophagic flux in ARPE-19.

Fig. 2. Acetylation stiffens primary cilia.

EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed.

The CREBBP-modulated network is enriched in signaling pathways upregulated in the light zone (LZ). The CREBBP-modulated network is enriched in signaling.

Histones acetylation is decreased in H3K9 and H3K27 in chronically mtDNA-depleted cells because of decreased HAT activity. Histones acetylation is decreased.

Modification of the β-tubulin C-terminal tail does not affect the partitioning of tubulin between the soluble and polymerized fractions in response to.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.

Activity regulates eEF2K-dependent spine morphogenesis.

Fig. 3 Proteomic analysis and Western blot analysis of protein cargos of various EVs. Proteomic analysis and Western blot analysis of protein cargos of.

Spn promotes Rac1 GTPase activation in glioblastoma (GBM) cells.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Dysregulated NF-κB activation in Il1r8+/+/lpr and Il1r8−/−/lpr mice.

Fig. 3. KMS99220 inhibits activation of NFκB signaling via HO-1 induction. (A) BV2 cells were treated with 10 µM KMS99220 for 1 h, and then treated with.

Changes in signal transduction pathway induced by gefitinib.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

Effect of bevacizumab on the proliferation of A2780 cells.

Volume 92, Issue 2, Pages (October 2016)

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

related to Fig 1. Isolation and characterization of FAPs.

Presentation transcript:

The absence of the centrosome modulates actin filaments organization via FA signaling in a migrating cell. The absence of the centrosome modulates actin filaments organization via FA signaling in a migrating cell. (A) The bar graph (scaled according to the ANOVA P-value) is representative of the results from the Ingenuity Pathway Analysis of the proteomics data from the various FA fractions obtained from RPEp53−/−: RPEp53−/−SAS6−/− (gray) or RPEp53−/−: RPEp53−/−STIL−/−cells (black); this shows the most dysregulated canonical pathways that are involved in migratory signaling. (B) Collective modulation by the centrosome of the abundance of small GTPases pathway proteins in the FAs. Proteins are represented by boxes that are color-coded according to their statistical significance categories and the magnitude of their ratio (as is indicated below each diagram). A P-value < 0.05 (t test) is considered to indicate with high confidence a significant change. Of the proteins with P-value < 0.05, the proteins with centrosome dependence ratio > 1 were considered to undergo centrosome-dependent recruitment, whereas the proteins with centrosome dependence ratio < 1 were considered to have their recruitment inhibited by the centrosome. (C) FA fractions from RPEp53−/−, RPEp53−/−SAS6−/−, and RPEp53−/−STIL−/−cells were analyzed by Western blotting using antibodies against paxillin, Rac1, TRIO, GEF-H1, active integrin β1, and total integrin β1. Fold (WB) (RPEp53−/−: RPEp53−/−SAS6−/−: RPEp53−/−STIL−/−cells) indicates the fold enrichment of paxillin (1: 0.97 ± 0.12: 1.04 ± 0.18; n = 12 independent experiments), Rac1 (1: 1.40 ± 0.13: 2.11 ± 0.25; n = 22 independent experiments), TRIO (1: 1.51 ± 0.24: 1.53 ± 0.25; n = 10 independent experiments), GEF-H1 (1: 1.25 ± 0.10: 2.30 ± 0.29; n = 16 independent experiments), active integrin β1 (1: 2.89 ± 0.44: 3.68 ± 0.40; n = 11 independent experiments), and total integrin β1 (1: 2.11 ± 0.47: 2.82 ± 0.50; n = 9 independent experiments) in isolated FA fractions as determined by Western blotting (loaded with equal amount of total protein, data are mean ± SEM). Fold (MS) indicates fold enrichment of the indicated proteins in the various isolated FA fractions as determined by LC-MS/MS. (D) Confocal images of immunolocalized γ-tubulin (purple) and phalloidin (green) in RPEp53−/−, RPEp53−/−SAS6−/−, and RPEp53−/−STIL−/−cells. Scale bars, 20 μm. Hung-Wei Cheng et al. LSA 2019;2:e201800135 © 2019 Cheng et al.