Volume 14, Issue 5, Pages (June 2004)

Slides:

Advertisements

Similar presentations

Volume 75, Issue 12, Pages (June 2009)

Advertisements

Volume 32, Issue 1, Pages (October 2008)

BRCA1 Is Associated with a Human SWI/SNF-Related Complex

Structural Changes in TAF4b-TFIID Correlate with Promoter Selectivity

Volume 36, Issue 5, Pages (December 2009)

Volume 20, Issue 6, Pages (June 1998)

Volume 41, Issue 5, Pages (March 2011)

PHAPI, CAS, and Hsp70 Promote Apoptosome Formation by Preventing Apaf-1 Aggregation and Enhancing Nucleotide Exchange on Apaf-1 Hyun-Eui Kim, Xuejun.

Hirotaka Matsui, Hiroya Asou, Toshiya Inaba Molecular Cell

Volume 22, Issue 2, Pages (February 2014)

Volume 22, Issue 3, Pages (May 2006)

by Guang Yang, Shu-Ching Huang, Jane Y. Wu, and Edward J. Benz

Volume 3, Issue 1, Pages (January 1999)

Yan Jiang, Mingyi Liu, Charlotte A. Spencer, David H. Price

Volume 38, Issue 4, Pages (May 2010)

Volume 31, Issue 3, Pages (August 2008)

Volume 26, Issue 5, Pages (June 2007)

Volume 19, Issue 6, Pages (September 2005)

Kuang-Lei Tsai, Chieri Tomomori-Sato, Shigeo Sato, Ronald C

Replication-Independent Histone Deposition by the HIR Complex and Asf1

Volume 116, Issue 1, Pages (January 2004)

ATP-Dependent Positive Supercoiling of DNA by 13S Condensin: A Biochemical Implication for Chromosome Condensation Keiji Kimura, Tatsuya Hirano Cell

Pnut is phosphorylated during early Drosophila embryogenesis.

A A B C D Fraction number b MCF7 lysate fraction A B C D A B C D GST-p851 GST Kinase assay GST-p851.

Volume 24, Issue 6, Pages (December 2006)

Volume 117, Issue 3, Pages (April 2004)

The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches Dirk Winter, Alexandre.

Volume 38, Issue 4, Pages (May 2010)

MCM9 Is Required for Mammalian DNA Mismatch Repair

Volume 97, Issue 1, Pages (April 1999)

Volume 123, Issue 2, Pages (October 2005)

Volume 20, Issue 1, Pages 9-19 (October 2005)

Volume 3, Issue 3, Pages (March 1999)

A TRF1:BRF Complex Directs Drosophila RNA Polymerase III Transcription

Volume 38, Issue 5, Pages (June 2010)

p53 Protein Exhibits 3′-to-5′ Exonuclease Activity

Daniel F. Bogenhagen, Dwight W. Martin, Antonius Koller

DNA Topoisomerase I and PC4 Can Interact with Human TFIIIC to Promote Both Accurate Termination and Transcription Reinitiation by RNA Polymerase III

Targeted Proteomic Study of the Cyclin-Cdk Module

Mediator–Nucleosome Interaction

Sukhyun Kang, Megan D. Warner, Stephen P. Bell Molecular Cell

Volume 67, Issue 3, Pages e5 (August 2017)

Volume 104, Issue 2, Pages (January 2001)

Volume 6, Issue 3, Pages (September 2000)

Volume 31, Issue 6, Pages (September 2008)

Dimitra J. Mitsiou, Hendrik G. Stunnenberg Molecular Cell

TopBP1 Activates the ATR-ATRIP Complex

Histone–GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells Teru Kanda, Kevin F. Sullivan, Geoffrey M. Wahl

Volume 32, Issue 5, Pages (December 2008)

Replication-Independent Histone Deposition by the HIR Complex and Asf1

Purification and identification of RECQ4 complex from human cells.

Volume 108, Issue 1, Pages (January 2015)

Volume 2, Issue 2, Pages (August 1998)

Volume 61, Issue 2, Pages (January 2016)

Human Pre-mRNA Cleavage Factor Im Is Related to Spliceosomal SR Proteins and Can Be Reconstituted In Vitro from Recombinant Subunits Ursula Rüegsegger,

Yan Jiang, Mingyi Liu, Charlotte A. Spencer, David H. Price

Volume 37, Issue 3, Pages (February 2010)

Volume 45, Issue 1, Pages (January 2012)

Salivary Lactoferrin Is Recognized by the Human Herpesvirus-8

Volume 29, Issue 4, Pages (February 2008)

Volume 9, Issue 1, Pages (January 2002)

4E-BP is present in an 80 kDa complex in unfertilized eggs.

Fig. 1. Stomatin expression in 3T3-L1 adipocytes

Volume 2, Issue 6, Pages (December 1998)

Volume 22, Issue 3, Pages (May 2006)

Epitope-tagged rice RPL18 assembles into functional ribosomes that can be purified by TRAP. (A) Confirmation of RPL18 assembly into ribosomes. Epitope-tagged.

Volume 41, Issue 4, Pages (February 2011)

Volume 3, Issue 1, Pages (January 1999)

Volume 123, Issue 2, Pages (October 2005)

Human Argonaute2 Mediates RNA Cleavage Targeted by miRNAs and siRNAs

Presentation transcript:

Volume 14, Issue 5, Pages 685-691 (June 2004) A Set of Consensus Mammalian Mediator Subunits Identified by Multidimensional Protein Identification Technology Shigeo Sato, Chieri Tomomori-Sato, Tari J Parmely, Laurence Florens, Boris Zybailov, Selene K Swanson, Charles A.S Banks, Jingji Jin, Yong Cai, Michael P Washburn, Joan Weliky Conaway, Ronald C Conaway Molecular Cell Volume 14, Issue 5, Pages 685-691 (June 2004) DOI: 10.1016/j.molcel.2004.05.006

Figure 1 Consensus Mammalian Mediator Subunits Identified by MudPIT (A) Mammalian Mediator subunits identified in different Mediator preparations. Mediator subunits detected in the TRAP/SMCC, ARC, DRIP, CRSP, PC2, mouse Mediator, and rat Mediator are indicated in red. Proteins not detected are indicated in green. (B) Mammalian Mediator subunits identified by MudPIT analyses of Mediator preparations from FLAG-Nut2 (MED10), FLAG-Med25 (MED9), FLAG-Intersex (MED29), FLAG-LCMR1 (MED19), or FLAG-MED28-expressing HeLa cell lines. A portion of the MudPIT data set for the FLAG-Med25 Mediator preparation was described elsewhere (Tomomori-Sato et al., 2003). (C) Analyses of Mediator preparations following phosphocellulose chromatography. P0.5 and P1.0 correspond to fractions eluted from phosphocellulose from 0.3 to 0.5 M KCl and from 0.5 to 1.0 M KCl, respectively. In (B) and (C), Mediator subunits detected by MudPIT are indicated in red with the percent amino acid sequence coverage; proteins not detected are indicated in green. “*,” a single potential TRAP230 peptide was identified by MudPIT in the P1.0 fraction but could not be unambiguously assigned to TRAP230. Molecular Cell 2004 14, 685-691DOI: (10.1016/j.molcel.2004.05.006)

Figure 2 SDS Polyacrylamide Gel of Mediator Preparations Analyzed by MudPIT Aliquots of anti-FLAG agarose eluates prepared from equivalent numbers of parental HeLa cells and HeLa cells stably expressing FLAG-Nut2 (MED10) [f:Nut2 (MED10)], FLAG-Intersex (MED29) [f:Intersex (MED29)], FLAG-Med25 (MED9) [f:Med25 (MED9)], FLAG-LCMR1 (MED19) [f:LCMR1 (MED19)], FLAG-MED28 (f:MED28), or FLAG-CRSP70 (MED26) [f:CRSP70 (MED26)] were subjected to SDS polyacrylamide gel electrophoresis in a 4%–20% gradient gel visualized by silver staining. The positions and relative molecular masses in kDa of protein size standards for lanes 1–6 are indicated on the left. Lane 9 shows size markers for lanes 7 and 8. Molecular Cell 2004 14, 685-691DOI: (10.1016/j.molcel.2004.05.006)

Figure 3 Pol II Subunits Identified in Mediator Preparations by MudPIT Pol II subunits detected by MudPIT are indicated in red with the percent amino acid sequence coverage; polymerase subunits not detected are indicated in green. Molecular Cell 2004 14, 685-691DOI: (10.1016/j.molcel.2004.05.006)

Figure 4 TRAP230 and Cdk8 Are Underrepresented in Mediator Purified through CRSP70 Aliquots of anti-FLAG agarose eluates from HeLa cells expressing FLAG-Nut2 (MED10) [f:Nut2 (MED10)] or FLAG-CRSP70 (MED26) [f:CRSP70 (MED26)] were analyzed by immunoblotting using antibodies against MED8 (Brower et al., 2002), TRAP230 (MED12) (S-19, Santa Cruz Biotechnology), CDK8 (D-9, Santa Cruz Biotechnology), or Rpb1 (N-20, Santa Cruz Biotechnology). Molecular Cell 2004 14, 685-691DOI: (10.1016/j.molcel.2004.05.006)