1.4 Membrane transport Essential idea: Membranes control the composition of cells by active and passive transport. The background image is a piece of artwork inspired by the complexity of an E. Coli. Complexity in cell structure is much greater still in Eukaryotes and this only possible through the compartmentalisation and the selective transport membranes allow. Edited and Revised by Eran Earland Acknowledgements to Chris Paine https://bioknowledgy.weebly.com/ and BioNinja http://onlinelibrary.wiley.com/doi/10.1002/bmb.20345/full#fig2

Understandings, Applications and Skills Statement Guidance 1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. 1.4.U2 The fluidity of membranes allows materials to be taken into cells by endocytosis or released by exocytosis. 1.4.U3 Vesicles move materials within cells.   1.4.A1 Structure and function of sodium–potassium pumps for active transport and potassium channels for facilitated diffusion in axons. 1.4.A2 Tissues or organs to be used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis. 1.4.S1 Estimation of osmolarity in tissues by bathing samples in hypotonic and hypertonic solutions. (Practical 2) Osmosis experiments are a useful opportunity to stress the need for accurate mass and volume measurements in scientific experiments.

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport.

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. Cellular membranes possess two key qualities: They are semi-permeable (only certain materials may freely cross – large and charged substances are typically blocked) They are selective (membrane proteins may regulate the passage of material that cannot freely cross) Movement of materials across a biological membrane may occur either actively or passively

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. Passive Transport Passive transport involves the movement of material along a concentration gradient (high concentration ⇒ low concentration) Because materials are moving down a concentration gradient, it does not require the expenditure of energy (ATP hydrolysis) There are three main types of passive transport: Simple diffusion – movement of small or lipophilic molecules (e.g. O2, CO2, etc.) Osmosis – movement of water molecules (dependent on solute concentrations) Facilitated diffusion – movement of large or charged molecules via membrane proteins (e.g. ions, sucrose, etc.)

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. http://www.phschool.com/science/biology_place/labbench/lab1/concepts.html http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/animation__how_diffusion_works.html

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport.

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. http://www.northland.cc.mn.us/biology/Biology1111/animations/transport1.html

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport.

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. What is osmosis? http://www.flickr.com/photos/luchilu/399970490/

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. Osmosis is the net movement of water molecules across a semi-permeable membrane from a region of low solute concentration to a region of high solute concentration (until equilibrium is reached) Water is considered the universal solvent – it will associate with, and dissolve, polar or charged molecules (solutes) Because solutes cannot cross a cell membrane unaided, water will move to equalize the two solutions At a higher solute concentration there are less free water molecules in solution as water is associated with the solute Osmosis is essentially the diffusion of free water molecules and hence occurs from regions of low solute concentration *** low solute concentration = higher water concentration so water is going DOWN its gradient and thus Osmosis is Passive http://www.flickr.com/photos/luchilu/399970490/

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. Osmosis may occur when there is a partially permeable membrane, such as a cell membrane. When a cell is submerged in water, the water molecules pass through the cell membrane from an area of low solute concentration (outside the cell) to one of high solute concentration (inside the cell)  (Wikipedia) http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html Aquaporin is an integral protein that, as it’s name suggests, acts as a pore in the membrane that speeds the movement of water molecules http://opm.phar.umich.edu/protein.php?pdbid=1sor

1.4.S1 Estimation of osmolarity in tissues by bathing samples in hypotonic and hypertonic solutions. (Practical 2) Osmolarity is a measure of solute concentration, as defined by the number of osmoles of a solute per litre of solution (osmol/L) Solutions may be loosely categorised as hypertonic, hypotonic or isotonic according to their relative osmolarity Solutions with a relatively higher osmolarity are categorised as hypertonic (high solute concentration ⇒ gains water) Solutions with a relatively lower osmolarity are categorised as hypotonic (low solute concentration ⇒ loses water) Solutions that have the same osmolarity are categorised as isotonic (same solute concentration ⇒ no net water flow)

1.4.S1 Estimation of osmolarity in tissues by bathing samples in hypotonic and hypertonic solutions. (Practical 2) Estimating Osmolarity - The osmolarity of a tissue may be interpolated by bathing the sample in solutions with known osmolarities The tissue will lose water when placed in hypertonic solutions and gain water when placed in hypotonic solutions Water loss or gain may be determined by weighing the sample before and after bathing in solution Tissue osmolarity may be inferred by identifying the concentration of solution at which there is no weight change (i.e. isotonic)

1.4.S1 Estimation of osmolarity in tissues by bathing samples in hypotonic and hypertonic solutions. (Practical 2) Estimation of osmolarity is a simple lab with many possible variations. http://www.saps.org.uk/secondary/teaching-resources/286-measuring-the-water-potential-of-a-potato-cell The two lab protocols shown suggest different ways of measuring the dependent variable. This is an ideal opportunity to practise and improve your understanding of the following IA criteria: Analysis Evaluation Communication http://www.nuffieldfoundation.org/practical-biology/investigating-effect-concentration-blackcurrant-squash-osmosis-chipped-potatoes n.b. estimation of osmolarity should be limited to statements such as “this tissue has an osmolarity equivalent to a 2% sucrose solution”. Molar concentrations maybe used in place of % and other solution such as sodium chloride maybe used in place of glucose.

1.4.A2 Tissues or organs to be used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis. Tissues or organs to be used in medical procedures must be kept in solution to prevent cellular desiccation This solution must share the same osmolality as the tissue / organ (i.e. isotonic) in order to prevent osmosis from occurring Uncontrolled osmosis will have negative effects with regards to cell viability: In hypertonic solutions, water will leave the cell causing it to shrivel (crenation) In hypotonic solutions, water will enter the cell causing it to swell and potentially burst (lysis) In plant tissues, the effects of uncontrolled osmosis are moderated by the presence of an inflexible cell wall In hypertonic solutions, the cytoplasm will shrink (plasmolysis) but the cell wall will maintain a structured shape In hypotonic solutions, the cytoplasm will expand but be unable to rupture within the constraints of the cell wall (turgor) http://commons.wikimedia.org/wiki/File:Osmotic_pressure_on_blood_cells_diagram.svg http://commons.wikimedia.org/wiki/File:Turgor_pressure_on_plant_cells_diagram.svg

The importance of osmotic control 1.4.A2 Tissues or organs to be used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis. The importance of osmotic control http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html http://commons.wikimedia.org/wiki/File:Osmotic_pressure_on_blood_cells_diagram.svg http://commons.wikimedia.org/wiki/File:Turgor_pressure_on_plant_cells_diagram.svg

The importance of osmotic control 1.4.A2 Tissues or organs to be used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis. The importance of osmotic control preventing damage to cells and tissues Common medical procedures in which an isotonic saline solution is useful: fluids introduction to a patient’s blood system via an intravenous drip, e.g for rehydration used to rinse wounds, skin abrasions etc. keep areas of damaged skin moist before applying skin grafts eye drops/wash frozen and used pack donor organs for transportation http://www.defenseimagery.mil/imageRetrieve.action?guid=8c9d5fade029a4f5a68fe667d1ae802ba9f30dd5&t=2

Facilitated Diffusion: 1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. Facilitated Diffusion: Large and polar molecules can’t get across the membrane via simple diffusion Transmembrane (polytopic) proteins recognise a particular molecule and help it to move across the membrane. The direction it moves is dependent on the concentration gradient. Watch the animation http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_facilitated_diffusion_works.html http://commons.wikimedia.org/wiki/File:Scheme_facilitated_diffusion_in_cell_membrane-en.svg

Facilitated diffusion is the passive movement of molecules across the cell membrane via the aid of a membrane protein It is utilized by molecules that are unable to freely cross the phospholipid bilayer (e.g. large, polar molecules and ions) This process is mediated by two distinct types of transport proteins – channel proteins and carrier proteins Carrier Proteins Integral glycoproteins which bind a solute and undergo a conformational change to translocate the solute across the membrane Carrier proteins will only bind a specific molecule via an attachment similar to an enzyme-substrate interaction Carrier proteins may move molecules against concentration gradients in the presence of ATP (i.e. are used in active transport) Carrier proteins have a much slower rate of transport than channel proteins (by an order of ~1,000 molecules per second) Channel Proteins Integral lipoproteins which contain a pore via which ions may cross from one side of the membrane to the other Channel proteins are ion-selective and may be gated to regulate the passage of ions in response to certain stimuli Channel proteins only move molecules along a concentration gradient (i.e. are not used in active transport) Channel proteins have a much faster rate of transport than carrier proteins

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. Active Transport Active transport involves the movement of materials against a concentration gradient (low concentration ⇒ high concentration) Because materials are moving against the gradient, it requires the expenditure of energy (e.g. ATP hydrolysis) There are two main types of active transport: Primary (direct) active transport – Involves the direct use of metabolic energy (e.g. ATP hydrolysis) to mediate transport Secondary (indirect) active transport – Involves coupling the molecule with another moving along an electrochemical gradient

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport.

1.4.U1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport.