Erik Melén, BSc, Anna Pomés, PhD, Lisa D. Vailes, MS, L

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A novel wheat gliadin as a cause of exercise-induced anaphylaxis

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Molecular cloning of Per a 1 and definition of the cross-reactive Group 1 cockroach allergens Erik Melén, BSc, Anna Pomés, PhD, Lisa D. Vailes, MS, L.Karla Arruda, MD, PhD, Martin D. Chapman, PhD Journal of Allergy and Clinical Immunology Volume 103, Issue 5, Pages 859-864 (May 1999) DOI: 10.1016/S0091-6749(99)70430-6 Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 1 Plaque immunoassay screening of the P americana cDNA clone. Upper panel , Comparison of IgE antibody binding by using sera from patients allergic to cockroaches with IgE antibody to Bla g 1; sera from patients with IgE antibody to Bla g 2, but not to Bla g 1; or sera from control patients. Lower panel , Plaque immunoassays with anti-Bla g 1 mAb 10A6 at 10 μg/mL (left, in duplicate ) or at 1 μg/mL (right ); anti-Bla g 2 mAb 8F4 was used at 10 μg/mL. Journal of Allergy and Clinical Immunology 1999 103, 859-864DOI: (10.1016/S0091-6749(99)70430-6) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 2 Plaque immunoassay with anti-Per a 1 mAb 02 to 08. Journal of Allergy and Clinical Immunology 1999 103, 859-864DOI: (10.1016/S0091-6749(99)70430-6) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 3 Alignment between Per a 1, Cr-PII, and mosquito protein ANG12. Amino acids in bold indicate homology with ANG12 (overall, 31%). The 14 amino acids that differ between Per a 1 and Cr-PII are indicated by asterisks. Journal of Allergy and Clinical Immunology 1999 103, 859-864DOI: (10.1016/S0091-6749(99)70430-6) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 4 SDS-PAGE analysis of recombinant Per a 1 expressed in Pichia pastoris . Lane 1, Molecular weight markers; lane 2, culture supernate from a rPer a 1 Pichia pastoris clone 3-1#3; lane 3, rPer a 1 purified from culture supernate which was passed over a Phenyl Sepharose column and eluted with water; lane 4, Phenyl Sepharose rPer a 1 further purified over a MonoQ HR5/5 HPLC column. Journal of Allergy and Clinical Immunology 1999 103, 859-864DOI: (10.1016/S0091-6749(99)70430-6) Copyright © 1999 Mosby, Inc. Terms and Conditions