Segregation of endolysosomal proteins in the Golgi complex.

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Segregation of endolysosomal proteins in the Golgi complex. Segregation of endolysosomal proteins in the Golgi complex. (a–f) HeLa cells coexpressing streptavidin–KDEL with combinations of the indicated reporter proteins were fixed 30 min after the addition of biotin and imaged by Airyscan microscopy. (a and d) Golgi complexes of representative cells. (b and e) Magnified views of box 1. (c and f) Magnified views of box 2 and plots of fluorescence intensity along the white dashed lines. (g and h) HeLa cells coexpressing streptavidin–KDEL with combinations of the indicated reporter proteins were imaged live by Airyscan microscopy. The top rows show Golgi complexes from representative cells. The middle rows show magnifications of the boxed region. The bottom row shows plots of fluorescence intensity along the white dashed lines. Bars, 1 µm. (i) Pearson’s coefficients (r) of data sets of which g and h are representative. Values were normalized to 1.0 at the first time point and are represented as mean ± SEM (n = 7 cells for each pair of cargos). ns, not significant; *, P < 0.1; **, P < 0.01; ***, P < 0.001. Times indicated in g–i are normalized to observable initiation of tubule budding, allowing comparative statistics. Yu Chen et al. J Cell Biol 2017;216:4141-4151 This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.