Custom Design of a GeXP Multiplexed Assay Used to Assess Expression Profiles of Inflammatory Gene Targets in Normal Colon, Polyp, and Tumor Tissue Janice.

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Custom Design of a GeXP Multiplexed Assay Used to Assess Expression Profiles of Inflammatory Gene Targets in Normal Colon, Polyp, and Tumor Tissue Janice E. Drew, Claus-Dieter Mayer, Andrew J. Farquharson, Pauline Young, Lawrence N. Barrera The Journal of Molecular Diagnostics Volume 13, Issue 2, Pages 233-242 (March 2011) DOI: 10.1016/j.jmoldx.2010.10.001 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Electropherograms showing quantitative multiplex GeXP-PCR of human colon using custom designed hInflamPlex. The sizes of detected amplicons in hInflamPlex assay of normal (A), polyp (B), and tumor (C) are shown. See Table 1 for a comprehensive list of fragment sizes of amplicons in hInflamPlex. The Journal of Molecular Diagnostics 2011 13, 233-242DOI: (10.1016/j.jmoldx.2010.10.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Relative gene expression levels in human normal, polyp, and tumor tissue generated using the GeXP hInflamPlex assay. Gene expression is normalized to internal reference genes in the hInflamPlex GAPDH (A) and B2M (B). The solid and dotted lines indicate significant differences in expression between normal and polyp and normal and tumor tissue, respectively. *P < 0.05. The Journal of Molecular Diagnostics 2011 13, 233-242DOI: (10.1016/j.jmoldx.2010.10.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 PCA of GAPDH- and B2M-normalized GeXP hInflamPlex and real-time data from seven human normal colon (N) and polyp (P) tissues. The tissue IDs shown on the PCA plots are listed in Table 1. GeXP hInflamPlex data normalized to GAPDH (A), real-time PCR data normalized to GAPDH (B), GeXP hInflamPlex data normalized to B2M (C), and real-time PCR data normalized to B2M (D). The Journal of Molecular Diagnostics 2011 13, 233-242DOI: (10.1016/j.jmoldx.2010.10.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions