College of Health Sciences, Korea University Lecture 10 Differential Count-Morphology Review (from Rodak Hematology) Kyung Jin Cho, PhD, Professor Emeritus chokj@korea.ac.kr Dept. of Medical Laboratory Science College of Health Sciences, Korea University

Segmented Neutrophil (분엽핵호중구) Maslak, P. ASH Image Bank 2008;2008:8-00131 2

Segmented Neutrophils  Band Segmented Neutrophils  Rodak Hematology 5th Ed. 2016, p153 Fig 12-5

Eosinophil *: MBP, major basic protein; ROS, reactive oxygen species; PAF, platelet activating factor 4

Maslak, P. ASH Image Bank 2008;2008:8-00127 Eosinophil (top) with classic red-orange globular granules in the cytoplasm Copyright ©2008 American Society of Hematology. Copyright restrictions may apply. Maslak, P. ASH Image Bank 2008;2008:8-00127 http://ashimagebank.hematologylibrary.org/ √

Basophil √ Fig 11-1 Abbas Basic immun 3rd Ed. 6

Monocyte Maslak, P. ASH Image Bank 2008;2008:8-00128 7

Lymphocyte Cytotoxic T / NK cell (CD8+ cells) 8 Maslak, P. 10/1/08. This image was originally published in ASH Image Bank. ASH Image Bank. © the American Society of Hematology." Cytotoxic T / NK cell (CD8+ cells) Fig 12-21 Keohane EM., et al., Rodak Hematology 5th Ed., 2015, p 163. Copyright © 2012, 2007, 2002, 1995 by Saunders, an imprint of Elsevier Inc. 8

Three lymphocytes Fig 12-20. Rodak Hematology 5th Ed. 2016, p163 A small resting lymphocyte(A) is stimulated by antigen and begins to form a medium and large lymphocyte (B). The nucleus reverts from a clumped to a delicate chromatin pattern with nucleoli (c). The cell is capable of dividing (blast transformation) to form effector cells or memory cells Fig 12-20. Rodak Hematology 5th Ed. 2016, p163

Lazarchick, J. ASH Image Bank 2010;2010:0-00098--00098 Reactive Lymphocytes Radial basophilia Lazarchick, J. ASH Image Bank 2010;2010:0-00098--00098 10