Volume 66, Pages S13-S19 (October 2004)

Slides:

Advertisements

Similar presentations

Volume 56, Issue 5, Pages (November 1999)

Advertisements

Figure 8. Colocalization of β-Arrestin2-GFP with Internalized wt and S(483–498)A Mutant PTHRs HEK-wt PTHR and HEK-S(483–498)A PTHR cells were transiently.

A novel SHP-1/Grb2–dependent mechanism of negative regulation of cytokine-receptor signaling: contribution of SHP-1 C-terminal tyrosines in cytokine signaling.

Toshiaki Monkawa, Tadashi Yoshida, Matsuhiko Hayashi, Takao Saruta

Large Hepatitis Delta Antigen Modulates Transforming Growth Factor-β Signaling Cascades: Implication of Hepatitis Delta Virus–Induced Liver Fibrosis

Activation of the Erythropoietin Receptor Is Not Required for Internalization of Bound Erythropoietin by Diana L. Beckman, Lilie L. Lin, Mary E. Quinones,

Takashi Tanaka, Michelle A. Soriano, Michael J. Grusby Immunity

The vacuolar-ATPase B1 subunit in distal tubular acidosis: novel mutations and mechanisms for dysfunction D.G. Fuster, J. Zhang, X.-S. Xie, O.W. Moe

Characterization of assembly of recombinant type IV collagen α3, α4, and α5 chains in transfected cell strains Takehiro Kobayashi, Makoto Uchiyama Kidney.

Volume 73, Issue 4, Pages (February 2008)

Volume 66, Pages S5-S12 (October 2004)

Volume 126, Issue 7, Pages (June 2004)

Volume 35, Issue 4, Pages (October 2011)

Volume 68, Issue 4, Pages (October 2005)

Volume 59, Issue 1, Pages (January 2001)

Volume 76, Issue 1, Pages (July 2009)

Volume 68, Issue 3, Pages (September 2005)

Volume 66, Issue 5, Pages (November 2004)

Volume 8, Issue 16, Pages (July 1998)

Istvan Arany, Judit K. Megyesi, Jane E.B. Reusch, Robert L. Safirstein

Volume 62, Issue 4, Pages (October 2002)

Volume 56, Issue 5, Pages (November 1999)

NRF2 Is a Major Target of ARF in p53-Independent Tumor Suppression

Angiotensin II-induced growth of vascular smooth muscle cells requires an Src- dependent activation of the epidermal growth factor receptor1 Dirk Bokemeyer,

17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production Naoko Kanda, Shinichi.

ASK1 Is Essential for JNK/SAPK Activation by TRAF2

Volume 3, Issue 6, Pages (June 1999)

Multiple PKCδ Tyrosine Residues Are Required for PKCδ-Dependent Activation of Involucrin Expression—a Key Role of PKCδ-Y311 Ling Zhu, Chaya Brodie, Sivaprakasam.

Volume 64, Issue 2, Pages (August 2003)

Toshiaki Monkawa, Tadashi Yoshida, Matsuhiko Hayashi, Takao Saruta

Volume 60, Issue 4, Pages (October 2001)

Robert L.S Perry, Maura H Parker, Michael A Rudnicki Molecular Cell

Volume 68, Issue 4, Pages (October 2005)

Arachidonic acid induces ERK activation via Src SH2 domain association with the epidermal growth factor receptor L.D. Alexander, Y. Ding, S. Alagarsamy,

MyD88: An Adapter That Recruits IRAK to the IL-1 Receptor Complex

Volume 59, Issue 5, Pages (May 2001)

Volume 93, Issue 5, Pages (May 1998)

Volume 62, Issue 3, Pages (September 2002)

Volume 79, Pages S20-S23 (April 2011)

Tetsuo Morioka, Jian Yao, Yasuhito Suzuki, Takashi Oite

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Volume 7, Issue 4, Pages (April 2001)

P. Harding, L. Balasubramanian, J. Swegan, A. Stevens, W.F. Glass

Lysine 63 Polyubiquitination of the Nerve Growth Factor Receptor TrkA Directs Internalization and Signaling Thangiah Geetha, Jianxiong Jiang, Marie W.

Volume 61, Issue 6, Pages (June 2002)

Volume 116, Issue 6, Pages (June 1999)

Volume 55, Issue 5, Pages (May 1999)

Volume 68, Issue 2, Pages (August 2005)

Temporal Regulation of Salmonella Virulence Effector Function by Proteasome- Dependent Protein Degradation Tomoko Kubori, Jorge E. Galán Cell Volume.

Volume 87, Issue 3, Pages (March 2015)

Volume 96, Issue 6, Pages (March 1999)

Volume 128, Issue 7, Pages (June 2005)

Volume 57, Issue 2, Pages (October 2000)

Volume 125, Issue 4, Pages (May 2006)

Mechanistic aspects of mineralocorticoid receptor activation

Volume 61, Issue 2, Pages (February 2002)

Volume 64, Issue 1, Pages (July 2003)

Shrestha Ghosh, Baohua Liu, Yi Wang, Quan Hao, Zhongjun Zhou

Latest findings in phosphate homeostasis

Volume 58, Issue 1, Pages (July 2000)

Volume 62, Issue 4, Pages (October 2002)

Volume 65, Issue 3, Pages (March 2004)

Volume 60, Issue 6, Pages (December 2001)

Volume 16, Issue 16, Pages (August 2006)

Volume 71, Issue 11, Pages (June 2007)

Volume 62, Issue 3, Pages (September 2002)

Volume 71, Issue 8, Pages (April 2007)

A Smad Transcriptional Corepressor

Volume 13, Issue 14, Pages (July 2003)

Gα12 and Gα13 Interact with Ser/Thr Protein Phosphatase Type 5 and Stimulate Its Phosphatase Activity Yoshiaki Yamaguchi, Hironori Katoh, Kazutoshi Mori,

Presentation transcript:

Volume 66, Pages S13-S19 (October 2004) PTH revisited12 Peter A. Friedman Kidney International Volume 66, Pages S13-S19 (October 2004) DOI: 10.1111/j.1523-1755.2004.09103.x Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 1 Parathyroid hormone (PTH) effects on [Ca2+]i in single proximal or distal tubule cells. At 2 minutes, 10 nmol/L PTH1-34 was added to the bath. Modified from55. Kidney International 2004 66, S13-S19DOI: (10.1111/j.1523-1755.2004.09103.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 2 Effects of PTH peptides on PTH1R internalization in proximal (PCT) and distal (DCT) kidney tubule cells. (A) PTH(1–34) internalizes the PTH1R in PCT and DCT cells, albeit with somewhat different kinetics. (B) PTH(7–34) avidly promotes PTH1R endocytosis in DCT cells, but has no effect on PCT cells. (C) Full-length PTH circulating peptides PTH(1–84) and PTH(7–84) exert effects comparable to their shorter synthetic analogues (i.e., both induce receptor sequestration in DCT cells). Kidney International 2004 66, S13-S19DOI: (10.1111/j.1523-1755.2004.09103.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 3 Na/H exchanger regulatory factor (NHERF) expression inhibits PTH/PTHrP (PTH1R) endocytosis in distal tubule cells. The results show the effect of 10-7 mol/L PTH(1–34) or PTH(7–34) on PTH1R internalization after 15 minutes in the absence or presence of NHERF1. **P < 0.01 vs. distal kidney tubule cells (DCT). Kidney International 2004 66, S13-S19DOI: (10.1111/j.1523-1755.2004.09103.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 4 Dominant-negative Na/H exchanger regulatory factor (dnNHERF) permits PTH(7–34) to internalize the PTH/PTHrP (PTH1R) in proximal tubule cells. The effect of 10-7 mol/L PTH(7–34) on PTH1R internalization is shown after 15-minute stimulation in cells transiently transfected with dnNHERF1 or empty vector. Kidney International 2004 66, S13-S19DOI: (10.1111/j.1523-1755.2004.09103.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 5 Coimmunoprecipitation of Na/H exchanger regulatory factor (NHERF1) with PTH1R(ETVM)-EGFP and PTH1R(ETVA)-EGFP. Human embryonic kidney (HEK)-293 cells transfected with the indicated constructs. Immunoprecipitation experiments were performed by incubating the cell lysates with an EGFP-specific polyclonal antibody. Immunoblotting was performed with an HA-specific monoclonal antibody (upper panel). The amount of immunoprecipitated EGFP-tagged receptor in each sample was verified by immunoblotting with the EGFP-specific polyclonal antibody (middle panel). The quantity of HA-NHERF1 present in each cell extract was evaluated by immunoblotting with the HA-specific monoclonal antibody (lower panel). Modified from48. See text for abbreviations. Kidney International 2004 66, S13-S19DOI: (10.1111/j.1523-1755.2004.09103.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 6 Na/H exchanger regulatory factor (NHERF1) effects on parathyroid hormone (PTH)-induced internalization in distal tubule cells expressing either the wild-type PTH/PTHrP (PTH1R) PDZ-recognition motif or the mutated ETVA PDZ-binding domain. The results depict the effect of 10-7 mol/L PTH(1–34) or PTH(7–34) for 15 minutes. **P < 0.01 vs. wild-type PTH1R. See text for abbreviations. Kidney International 2004 66, S13-S19DOI: (10.1111/j.1523-1755.2004.09103.x) Copyright © 2004 International Society of Nephrology Terms and Conditions