K. S. Ko, T. Kuwahara, L. Haehwa, Y. -J. Yoon, B. -J. Kim, K. -H

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RNA polymerase β-subunit gene (rpoB) sequence analysis for the identification of Bacteroides spp. K.S. Ko, T. Kuwahara, L. Haehwa, Y.-J. Yoon, B.-J. Kim, K.-H. Lee, Y. Ohnishi, Y.-H. Kook Clinical Microbiology and Infection Volume 13, Issue 1, Pages 48-54 (January 2007) DOI: 10.1111/j.1469-0691.2006.01553.x Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Schematic structure of rpoB DNA amplified with three different primer sets. (A) Positions of primers (BF and BR) used for the amplification of the 358-bp rpoB fragment for rpoB sequencing in this study. (B) Positions of Bacteroides genus-specific PCR primers (BacF and BR) used to amplify the 247-bp rpoB fragment. (C) Positions of Bacteroides fragilis species-specific PCR primers (Bfr-F and Bfr-R) used to amplify the 185-bp fragment. RifR indicates a rifampicin resistance-related region. The numbering of nucleotides and amino-acids is based on the rpoB sequence of Escherichia coli (GenBank accession no. V00339). Clinical Microbiology and Infection 2007 13, 48-54DOI: (10.1111/j.1469-0691.2006.01553.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 The rpoB tree of 19 reference strains, constructed by the neighbour-joining method in PAUP. The midpoint rooting method was used to root this tree. The branch lengths are proportional to the changes in the nucleotides. Branches supported by >50% of the bootstrap analyses (1000 replications) are indicated. The scale bar represents five substitutions/100 nucleotides. Clinical Microbiology and Infection 2007 13, 48-54DOI: (10.1111/j.1469-0691.2006.01553.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 The 16S rRNA gene tree of 19 reference strains constructed by the neighbour-joining method in PAUP (see Fig. 2). Clinical Microbiology and Infection 2007 13, 48-54DOI: (10.1111/j.1469-0691.2006.01553.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions