Norikatsu Mizumoto, Francis Hui, Dale Edelbaum, M

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Presentation transcript:

Differential Activation Profiles of Multiple Transcription Factors During Dendritic Cell Maturation Norikatsu Mizumoto, Francis Hui, Dale Edelbaum, M. Ryan Weil, Jonathan D. Wren, David Shalhevet, Hiroyuki Matsue, Lei Liu, Harold R. Garner, Akira Takashima Journal of Investigative Dermatology Volume 124, Issue 4, Pages 718-724 (April 2005) DOI: 10.1111/j.0022-202X.2005.23616.x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Phenotypic and functional plasticity of the XS106 dendritic cell (DC) line. XS106 DC were treated for 24 h with the indicated agents at the indicated concentrations. These samples were then tested for the surface expression or the secretion of the indicated molecules. Data are representative of two independent experiments, showing the mean±SD (n=3 per group). Asterisks or sharps indicate statistically significant (p<0.01) augmentation or inhibition as compared with untreated controls, respectively. Concentrations of test agents are all plotted in log scales (x-axis), and some outcome data sets are plotted in different scales (y-axis) for individual stimuli. Journal of Investigative Dermatology 2005 124, 718-724DOI: (10.1111/j.0022-202X.2005.23616.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Baseline activities of the tested transcription factors in XS106 dendritic cells (DC). XS106 DC were transduced with luciferase (Luc) reporter constructs containing the indicated cis-enhancer elements and examined for Luc expression 48 h later in the absence of added exogenous stimuli. Data are representative of six independent experiments, showing the means±SD (n=3) of relative Luc expression levels of individual channels compared with the negative control channel with no enhancers. Statistically significant differences above the negative control are indicated by asterisks (*p<0.01, **p<0.001). Journal of Investigative Dermatology 2005 124, 718-724DOI: (10.1111/j.0022-202X.2005.23616.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Stimulation-induced transcription factor activation profiles. (A) XS106 dendritic cells (DC) were transduced with the indicated luciferase (Luc) constructs and incubated for 24 h with interferon γ (IFNγ), adenosine triphosphate (ATP), lipopolysaccharide (LPS), necrotic keratinocytes, or croton oil (CrO). (B) XS106 DC and Pam 212 keratinocytes were transduced in parallel with the indicated Luc constructs and incubated for 24 h with ADP or CpG oligodeoxynucleotides. The data are representative of two independent experiments, showing the means±SD (n=3) of relative induction units (RIU) compared with the untreated controls. Asterisks indicate statistically significant induction compared with the untreated controls (*p<0.1, **p<0.01, ***p<0.001). Journal of Investigative Dermatology 2005 124, 718-724DOI: (10.1111/j.0022-202X.2005.23616.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Concentration dependency and time-course of stimulus-specific transcription factor activation. (A) XS106 dendritic cells (DC) were transduced with the indicated luciferase (Luc) constructs and incubated for 24 h with the reagents at the indicated concentrations (plotted in log scales). (B) XS106 DC were transduced with the indicated Luc constructs and incubated for the indicated periods with interferon γ (IFNγ) (10 ng per mL), adenosine triphosphate (ATP) (0.5 mM), lipopolysaccharide (LPS) (100 ng per mL), croton oil (CrO) (0.01%), or ADP (0.5 mM). The data in this figure are representative of two independent experiments, showing the means±SD (n=3) of relative induction units (RIU) compared with the untreated controls. Asterisks indicate statistically significant induction as compared with the untreated controls (*p<0.01, **p<0.001). Journal of Investigative Dermatology 2005 124, 718-724DOI: (10.1111/j.0022-202X.2005.23616.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Classification of dendritic cell (DC)-stimulatory signals based on transcription factor activation profiles. (A) Data shown are the luciferase induction profiles with the indicated channels (y-axis) following 24 h incubation with the indicated agents (x-axis). The color represents the relative induction units compared with the untreated controls, according to the shown scale. Data in this figure are representative of at least two independent experiments for each stimulus and hierarchical clustering was performed by using the GeneSpring program. (B) Differential impacts of Toll-like receptors (TLR) ligands and P2R ligands on TF activation profiles are illustrated. The line thickness represents the relative extent of activation of each TF. Journal of Investigative Dermatology 2005 124, 718-724DOI: (10.1111/j.0022-202X.2005.23616.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions