Multi-photon microscopic evaluation of saphenous vein endothelium and its preservation with a new solution, GALA Hemant S Thatte, PhD, Kunda S Biswas,

Slides:

Advertisements

Similar presentations

Does a Preservation Solution for Vascular Grafts Matter?

Advertisements

Acquired microvascular dysfunction in inflammatory bowel disease: loss of nitric oxide- mediated vasodilation Ossama A Hatoum, David G Binion, Mary F.

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Eddie L. Hoover, M. D. , Margery Ross, Ph. D. , Kazam Fani, M. D

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff Akio Koyama, MD, Kimihiro.

Impact of Cryopreservation on Extracellular Matrix Structures of Heart Valve Leaflets Katja Schenke-Layland, PhD, Navid Madershahian, MD, Iris Riemann,

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Acidosis-induced apoptosis in human and porcine heart

Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries Birgit Zatschler, Peter Dieterich, PhD, Bianca Müller,

Neutrophil Adherence to Activated Saphenous Vein and Mammary Endothelium After Graft Preparation Axel Schlitt, MD, Diethard Pruefer, MD, Ute Buerke,

David G. Cable, MD, James A. Caccitolo, MD, Eric A

Tissue factor and thrombin mediate myocardial ischemia-reperfusion injury Albert J Chong, MD, Timothy H Pohlman, MD, Craig R Hampton, MD, Akira Shimamoto,

Neovascularization and recurrent varicose veins: more histologic and ultrasound evidence André M van Rij, MD, FRACS, Gregory T Jones, PhD, Gerry B Hill,

VEGF Gene Delivery to Muscle

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Improvement of the cold storage of blood vessels with a vascular preservation solution. Study in porcine aortic segments Timo Wille, Herbert de Groot,

Endothelial cell injury induced by preservation solutions: a confocal microscopy study Francesco Alamanni, MD, Alessandro Parolari, MD, PhD, Rossana Visigalli,

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff Akio Koyama, MD, Kimihiro.

The coronary artery bypass conduit: I

Thomas V Bilfinger, MD, George B Stefano, PhD

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Implantation of an autologously endothelialized homograft

Cryopreserved Veins in Myocardial Revascularization: Possible Mechanism for Their Increased Failure Thomas V Bilfinger, MD, ScD, Alan R Hartman, MD,

Hemagglutinating virus of Japan-liposome–mediated gene transfer of endothelial cell nitric oxide synthase inhibits intimal hyperplasia of canine vein.

Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model Jian-Ming Li, MD, Mohammad.

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Novel Bioresorbable Vascular Graft With Sponge-Type Scaffold as a Small-Diameter Arterial Graft Tadahisa Sugiura, MD, PhD, Shuhei Tara, MD, PhD, Hidetaka.

Daniel J. Wong, MD, Daniel Y. Lu, MD, Clinton D

D. A. Lewis, PhD, R. C. Lowell, MD, R. A. Cambria, MD, P. C

Percutaneous transplantation of genetically-modified autologous fibroblasts in the rabbit femoral artery: a novel approach for cardiovascular gene therapy

Nanoparticle-mediated endothelial cell-selective delivery of pitavastatin induces functional collateral arteries (therapeutic arteriogenesis) in a rabbit.

Ambient pressure upregulates nitric oxide synthase in a phosphorylated-extracellular regulated kinase– and protein kinase C–dependent manner Angela G.

Inhibitory effect of photooxidation on intimal and medial thickening of saphenous vein Jyotirmay Chanda , MD, Ryosei Kuribayashi, MD, Ke-Xiang Liu, MD,

Expression and Function of Endothelial Nitric Oxide Synthase Messenger RNA and Protein Are Higher in Internal Mammary Than in Radial Arteries Guo-Wei.

Contractile smooth muscle cell apoptosis early after saphenous vein grafting Evelio Rodriguez, MD, Erica H Lambert, BS, Michael G Magno, PhD, John D Mannion,

Endothelial nitric oxide synthase affects both early and late collateral arterial adaptation and blood flow recovery after induction of hind limb ischemia.

Favorable scanning electron microscopic findings of stapled saphenous vein–carotid artery anastomoses Mohammad Bashar Izzat, Anthony P.C Yim, MD, Kenny.

Pharmacology of coronary artery bypass grafts

Viability and volume of in situ bovine articular chondrocytes—changes following a single impact and effects of medium osmolarity Dr Peter G. Bush, Ph.D.,

Annette Ebner, PhD, David M. Poitz, PhD, Antje Augstein, PhD, Ruth H

Mark F Berry, MD, Y.Joseph Woo, MD The Annals of Thoracic Surgery

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Gabriele Di Luozzo, MD, Jaya Bhargava, PhD, Richard J. Powell, MD

Effect of skeletonization of the internal thoracic artery on vessel wall integrity Mario Gaudino, MD, Amelia Toesca, PhD, Stefania Lucia Nori, MD, Franco.

Addition of Calcium to Euro-Collins Solution Is Essential for 24-Hour Preservation of the Vasculature Richard Ingemansson, MD, PhD, Ramunas Bolys, MD,

The effects of ginsenoside Rb1 on endothelial damage and ghrelin expression induced by hyperhomocysteine Zhiwei Xu, PhD, Taohua Lan, MD, Weikang Wu,

Xiao J. Feng, MD, PhDa, Cor E. J. Van Hove, b, Paul J

Comparable patencies of the radial artery and right internal thoracic artery or saphenous vein beyond 5 years: Results from the Radial Artery Patency.

Thrombolysis for experimental deep venous thrombosis maintains valvular competence and vasoreactivity Jeffrey M. Rhodes, MD, Jae-Sung Cho, MD, Peter.

Acquired microvascular dysfunction in inflammatory bowel disease: loss of nitric oxide- mediated vasodilation Ossama A Hatoum, David G Binion, Mary F.

Nilima Shukla, PhD, Gianni D. Angelini, FRCS, Jamie Y. Jeremy, PhD

Nitric Oxide Synthase and Adenylyl and Guanylyl Cyclase Activity in Porcine Interposition Vein Grafts Jamie Y Jeremy, PhD, Michael R Dashwood, PhD, Maureen.

Retaining perivascular tissue of human saphenous vein grafts protects against surgical and distension-induced damage and preserves endothelial nitric.

Pharmacologic relaxation of vein grafts is beneficial compared with pressure distention caused by upregulation of endothelial nitric oxide synthase and.

Laki J. Rousou, MS, MD, Kristin B

Surgical Preparation Abolishes Endothelium-Derived Hyperpolarizing Factor-Mediated Hyperpolarization in the Human Saphenous Vein Jian-An Yang, MD, Guo-Wei.

Diabetes mellitus and experimental vein graft structure and function

Preseeding with autologous fibroblasts improves endothelialization of glutaraldehyde- fixed porcine aortic valves Helmut Gulbins, MD, Angelika Goldemund,

Pressure distension stimulates the expression of endothelial adhesion molecules in the human saphenous vein graft Massimo Chello, MD, Pasquale Mastroroberto,

Altered Oxidative Stress Responses and Increased Type I Collagen Expression in Bicuspid Aortic Valve Patients Julie A. Phillippi, PhD, Michael A. Eskay,

Cigarette smoke inhibits endometrial epithelial cell proliferation through a nitric oxide– mediated pathway Omid Khorram, M.D., Ph.D., Guang Han, M.D.,

Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production Michael R. Dashwood, PhD, Kay Savage, PhD, Audrey Dooley,

How Cells Tiptoe on Adhesive Surfaces before Sticking

William C. Quist, MD, PhD, Frank W. LoGerfo, MD

Panagiotis Kougias, MD, Hong Chai, MD, PhD, Peter H. Lin, MD, Alan B

Prevention of stenosis after vascular reconstruction: Pharmacologic control of intimal hyperplasia—A review Alexander W. Clowes, MD, Michael A. Reidy,

Presentation transcript:

Multi-photon microscopic evaluation of saphenous vein endothelium and its preservation with a new solution, GALA Hemant S Thatte, PhD, Kunda S Biswas, MD, Samer F Najjar, MD, Vladimir Birjiniuk, MD, Michael D Crittenden, MD, Thomas Michel, MD, PhD, Shukri F Khuri, MD The Annals of Thoracic Surgery Volume 75, Issue 4, Pages 1145-1152 (April 2003) DOI: 10.1016/S0003-4975(02)04705-7

Fig 1 This figure illustrates changes in structural integrity of vein segments stored in different preservation solutions. Vein segments were stored in heparinized lidocaine saline (HLS), autologous heparinized blood (AHB), tissue culture medium (TCM) for 60 minutes; and in Hank’s balanced salt solution (HBSS) and the newly designed glutathione, ascorbic acid, and L-arginine (GALA) preservation solution for a total of 24 hours. Green fluorescence indicates cell viability; red fluorescence indicates compromised cells. Extensive cell membrane damage and compromised endothelial cell integrity in the vessels was observed after short-term storage in HLS, AHB, and TCM. Cell viability was well preserved in veins during short-term storage in HBSS but resulted in cell death upon extended storage. In contrast, endothelial cells remained viable in vessels preserved in GALA solution throughout the extended time of storage. Representative image, at ×400 magnification, n = 9. (EC = endothelial cells.) The Annals of Thoracic Surgery 2003 75, 1145-1152DOI: (10.1016/S0003-4975(02)04705-7)

Fig 2 Transverse sections of the saphenous veins imaged using multiphoton microscopy in transmission mode. The segments were imaged using XYZ scanning ∼100-μm deep from the site of excision. The vessel lumen, endothelial cell layer, and smooth muscle layer are clearly visible. Endothelial and smooth muscle cell damage was observed in segments stored for 60 minutes in heparinized lidocaine saline (HLS) and autologous heparinized blood (AHB). Structural integrity of the vessel was well preserved in vessels stored in glutathione, ascorbic acid, and L-arginine (GALA). Representative image at ×400 magnification, n = 9. The Annals of Thoracic Surgery 2003 75, 1145-1152DOI: (10.1016/S0003-4975(02)04705-7)

Fig 3 Bradykinin mediated calcium mobilization and nitric oxide generation in saphenous veins stored in glutathione, ascorbic acid, and L-arginine. Segments (similar to those illustrated in Fig 2) were labeled with calcium orange and diaminofluorescein, stimulated with bradykinin and imaged using multiphoton microscopy. A robust two- to threefold increase in calcium (red) and nitric oxide (green) fluorescence signal in the endothelial region was observed 10 minutes after bradykinin stimulation, that found to colocalize (orange-yellow fluorescence). Representative image of nine independent experiments. The Annals of Thoracic Surgery 2003 75, 1145-1152DOI: (10.1016/S0003-4975(02)04705-7)

Fig 4 Vein segments were stored in preservative solutions for various time points, then incubated with diaminofluorescein (DAF) to measure nitric oxide generation. The integrated DAF fluorescence intensity in the endothelial region of each vein was quantitated using multi-photon microscopy after a 10-minute treatment with bradykinin (10 μmol/L) and was then normalized to the fluorescence intensity measured before the drug treatment (dashed line = 1). Each bar represents the mean ± SEM of independent experiments (n = 9). Nitric oxide generation was severely impaired in veins stored in heparinized lidocaine saline (shaded bars), autologous heparinized blood (black-spotted bars), and tissue culture medium (white-spotted bars). Nitric oxide production was maintained in vessels during short-term storage in Hank’s balanced salt solution (black bars); however, a temporal decrease in endothelial nitric oxide synthase (eNOS) activity was observed. In contrast, eNOS activation and nitric oxide generation was well preserved in vessels stored in glutathione, ascorbic acid, and L-arginine (GALA) solution (white bars). A sustained increase in nitric oxide production was observed during extended storage of the vessels in GALA. The Annals of Thoracic Surgery 2003 75, 1145-1152DOI: (10.1016/S0003-4975(02)04705-7)

Fig 5 In-depth imaging of a saphenous vein segment stored in GALA solution using multi-photon microscopy. The vein was imaged at 4-μm steps along the Z axis, and the figure is a representative montage of two-dimensional images taken at depths mentioned in the figure. Green fluorescence indicates living cells; red fluorescence indicates dead cells. The smooth muscle cell layer (SMC; adventitia/media) is identifiable starting at ∼4 μm; vessel lumen and endothelial cell layer becomes clearly visible at ∼120 μm. This figure illustrates that the cell viability was well maintained throughout the vessel stored in GALA for 1440 minutes. ×400 magnification. The Annals of Thoracic Surgery 2003 75, 1145-1152DOI: (10.1016/S0003-4975(02)04705-7)