Pinopodes are present in Lif null and Hoxa10 null mice

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Presentation transcript:

Pinopodes are present in Lif null and Hoxa10 null mice Claire E. Quinn, M.Sc., Jacqui Detmar, M.Sc., Robert F. Casper, M.D. Fertility and Sterility Volume 88, Issue 4, Pages 1021-1028 (October 2007) DOI: 10.1016/j.fertnstert.2006.11.157 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Pinopode (∼7-μm diameter) in the ICR mouse endometrium, surrounded by smaller microvilli and blebs and by pinopodes. (B) Cartoon of morphological differences between pinopodes (P) extending from the apical surface of luminal epithelium cells in the rat (R), mouse (M), and human (H), as seen during their respective windows of receptivity. A and B, bar = 5 μm. Quinn. Pinopodes in the rodent endometrium. Fertil Steril 2007. Fertility and Sterility 2007 88, 1021-1028DOI: (10.1016/j.fertnstert.2006.11.157) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Mean (±SEM; n = 3 for each time point) pinopode score vs. day of pregnancy in ICR (white bars), Lif null (gray bars), and Hoxa10 null (black bars) mice. The day on which embryo implantation occurs is circled. (B) Scanning EM pictures of pinopodes on day 4.5 of pregnancy in intact ICR, Lif null, and Hoxa10 null mice. For all B subpanels, bar = 10 μm. Quinn. Pinopodes in the rodent endometrium. Fertil Steril 2007. Fertility and Sterility 2007 88, 1021-1028DOI: (10.1016/j.fertnstert.2006.11.157) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Mean (±SEM; n = 3 for each time point) pinopode score vs. day of pregnancy in Sprague-Dawley rats. The day of embryo implantation is circled. Bars with the same superscript letters are not statistically different from each other (P>.05). (B) Scanning EM pictures of pinopodes on day 5 of pregnancy. (B, i) Bar = 20 μm; (B, ii) bar = 5 μm. Quinn. Pinopodes in the rodent endometrium. Fertil Steril 2007. Fertility and Sterility 2007 88, 1021-1028DOI: (10.1016/j.fertnstert.2006.11.157) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 4 (A) Mean (±SEM; n = 3 for each time point) pinopode score vs. number of days after OVX in ICR mice. Bars with the same superscript letters are not statistically different from each other (P>.05). (B) Scanning EM pictures of pinopodes and macropodes on day 50 after OVX. Bar = 100 μm. Quinn. Pinopodes in the rodent endometrium. Fertil Steril 2007. Fertility and Sterility 2007 88, 1021-1028DOI: (10.1016/j.fertnstert.2006.11.157) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 5 (A) Mean (±SEM; n = 3 for each time point) pinopode score vs. day of treatment (vehicle [black bars], mid-dose E2 [gray bars], and high-dose E2 [white bars]) in OVX ICR mice (on days 5 and 10 of treatment and 5 d after withdrawal from E2 [day 15]). Bars with the same superscript letters are not statistically different from each other (P>.05). (B, i) Scanning EM pictures on day 10 of mid-dose E2 (bar =1 mm) and (B, ii) day 10 of vehicle (bar = 100 μm). Quinn. Pinopodes in the rodent endometrium. Fertil Steril 2007. Fertility and Sterility 2007 88, 1021-1028DOI: (10.1016/j.fertnstert.2006.11.157) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 6 Pinopode-like structures (∼5-μm diameter) on the apical surface of the trachea in the ICR mouse. Bar = 10 μm. Quinn. Pinopodes in the rodent endometrium. Fertil Steril 2007. Fertility and Sterility 2007 88, 1021-1028DOI: (10.1016/j.fertnstert.2006.11.157) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions