3D model and in vivo live imaging of dermal wound repair

Slides:

Advertisements

Similar presentations

Date of download: 6/3/2016 Copyright © 2016 SPIE. All rights reserved. (a) Confocal microscope images of human adipose-derived stem cells (hASCs) labeled.

Advertisements

Role of TNF in fracture healing in vivo Addition of rhTNF at the fracture site on days 0 and 1 led to augmented healing, indicated by increased % callus.

Tuning the response of the DPI

DNA‐anchored LacI–CFP–Luciferase aggregates capture misfolded cytosolic proteins. DNA‐anchored LacI–CFP–Luciferase aggregates capture misfolded cytosolic.

A culture‐induced TE‐like subpopulation

Periodic changes in distribution of ERK–GFP fusion protein in cells after stimulation with EGF. (A) Confocal image of cells expressing ERK–GFP both before.

Glycoprotein Nonmelanoma Clone B Regulates the Crosstalk between Macrophages and Mesenchymal Stem Cells toward Wound Repair Bing Yu, Talib Alboslemy,

Analysis of mutational effects of parameters on the phenotype (i. e

Sensitivity of RNA‐seq.

Clonal expansion of sporadically induced K‐rasG12D in Lgr5hi cells Confocal scanning of the bottom of small intestinal crypts at indicated time points.

The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.

Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.

HIS-24K14me1 interacts with HPL-1.

Ectodysplasin A Pathway Contributes to Human and Murine Skin Repair

Dose response of pAGA1 and pFIG1 induction

Model training of generalized Lotka–Volterra (gLV) to time‐resolved measurements of monospecies and pairwise assemblages Model training of generalized.

cuf2Δ and fzr1Δ cells show a defect in meiotic termination.

TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.

Chd5 deficiency in NSCs leads to premature activation.

Noise in gene expression depends on the promoter class and on TF dynamics. Noise in gene expression depends on the promoter class and on TF dynamics. (A,

Characterization of promoters relative to pAGA1

Dynamics and variability of SMAD2 signaling in single cells

TFEB stimulates lysosomal–autophagosomal fusion and clearance

Heat maps showing global relative growth phenotype and comparison between measured and predicted values. Heat maps showing global relative growth phenotype.

Dermal architecture is defined by an inverse correlation between fibroblast proliferation and ECM deposition Dermal architecture is defined by an inverse.

RNAi causes widespread changes in cell population context leading to predictable changes in virus infection. RNAi causes widespread changes in cell population.

Alignment time for Clustal Omega (red), MAFFT (blue), MUSCLE (green) and Kalign (purple) against the number of sequences of HomFam test sets. Alignment.

Mutants of the Group III have differentially impaired induction of pAGA1 and pFIG1 Mutants of the Group III have differentially impaired induction of pAGA1.

Dynamic map of the Nap1p/Kcc4p interaction.

Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.

Lack of Collagen VI Promotes Wound-Induced Hair Growth

Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+

A Murine Living Skin Equivalent Amenable to Live-Cell Imaging: Analysis of the Roles of Connexins in the Epidermis Eve E. Kandyba, Malcolm B. Hodgins,

Tropomyosin Regulates Cell Migration during Skin Wound Healing

Fibroblast proliferation and ECM changes during wound healing

Patient organoids respond more diverse to drugs and with lower therapeutic potential than 2D cultured patient cells Patient organoids respond more diverse.

Aberrant expression of filaggrin in SASP−/− hairless mice.

Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo.

Shared components define the ‘ancient’ phagosome.

Fate of Prominin-1 Expressing Dermal Papilla Cells during Homeostasis, Wound Healing and Wnt Activation Grace S. Kaushal, Emanuel Rognoni, Beate M. Lichtenberger,

Knock‐in of PP7 stem‐loop sequences provides visualization of estrogen‐mediated transcription from the endogenous GREB1 locus in living cells (see also.

Volume 1, Issue 1, Pages (June 2013)

Comparison of nuclear surface area of HeLa and RKO cells

Effects of ERK–GFP expression levels and cell density on ERK phosphorylation and oscillations. Effects of ERK–GFP expression levels and cell density on.

Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal.

Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.

Proliferative advantage depends on environmental dynamics

Stage‐dependent diurnal transcript changes.

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Cluster analysis of all identified models.

Protein interactions at the nuclear pore.

Characterising dermis expansion and gene expression changes during mouse development (related to Fig 1)‏ Characterising dermis expansion and gene expression.

In vivo imaging of the wound bed at later time points (related to Fig 7)‏ In vivo imaging of the wound bed at later time points (related to Fig 7) A–CRepresentative.

Antisense expression associates with larger gene expression variability. Antisense expression associates with larger gene expression variability. (A–D)

Experimental validation of predicted endocytosis functions in human.

Characterization of immunosorted NCAM1+ subpopulation A

Global and focused views of human interaction map.

Remodeling of the mitochondrial and extracellular proteome during Caenorhabditis elegans aging Remodeling of the mitochondrial and extracellular proteome.

Competence initiation during the progression to spore formation.

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

(A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). (A) Observed.

Kinetics of GREB1 induction by estrogen in single cells (see also Fig EV4 and Movie EV3)‏ Kinetics of GREB1 induction by estrogen in single cells (see.

Pericytes enhance LAMA5 deposition and assembly of the basement membrane and hemi-desmosomes in OCs. Pericytes enhance LAMA5 deposition and assembly of.

Volume 17, Issue 9, Pages (November 2016)

Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.

Remodelling of adult dermis originates from a population of fibroblasts in the hair follicle junctional zone. Remodelling of adult dermis originates from.

Y. Albert Pan, Joshua R. Sanes Journal of Investigative Dermatology

Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.

Interaction of aggregates with CAF network structures.

Fig. 3 4n hybrids undergo bipolar and tripolar mitosis.

Presentation transcript:

3D model and in vivo live imaging of dermal wound repair 3D model and in vivo live imaging of dermal wound repair A(x,y) Cross‐section of the computational simulation of wound healing in adult dermis. Please refer to associated Movie EV4.B–DLive imaging of adult PDGFRαH2BEGFP back skin during wound healing. (B) Experimental design of in vivo imaging of wound healing. (C) Representative wound bed Z‐stack stitched maximum projections of the entire wound bed (upper panel) showing fibroblasts (green) and collagen with second harmonic generation (SHG) in purple at indicated time points after wounding and before live imaging. Dotted line indicates wound edge, and boxed area indicates the position of the magnified area below. (D) Representative time‐lapse images of adult wound bed at PW4, where Z‐directional movement is visualised by pseudo‐colouring the fibroblast nuclei (red, movement towards the basement membrane; blue, movement away from the basement membrane). Dotted line indicates wound edge. Please refer to associated Movie EV6 which shows fibroblasts actively migrating into the wound bed.E–HIn vivo lineage tracing of upper (Blimp1Cre) and lower (Dlk1CreER) dermal fibroblasts during wound healing. (E) Experimental strategy for in vivo lineage tracing. (F) Immunofluorescence image of Blimp1Cre × Confetti (upper panels) and Dlk1CreER × Confetti (lower panel) back skin inside and outside the wound bed at 10 days postwounding. Colours show YFP (green), RFP (red) and CFP (blue) with bright field image overlaid. (G) Clone size quantification of Blimp1Cre‐labelled fibroblasts inside and outside the wound bed at 10 days postwounding (n = 400 clones of four biological replicates). (H) Clone size quantification of Dlk1CreER‐labelled fibroblasts inside and outside the wound bed at 10 days postwounding (n = 300 clones of two biological replicates).Data information: Scale bars, 500 μm (C); 100 μm (D, F). Emanuel Rognoni et al. Mol Syst Biol 2018;14:e8174 © as stated in the article, figure or figure legend